GeneTex

  • Country / Location Selection

United States (US)

Calpain 5 antibody [HL2432]

Recombinant antibody Orthogonal
Anti-Calpain 5 antibody [HL2432] used in Western Blot (WB). GTX638748
Anti-Calpain 5 antibody [HL2432] used in Western Blot (WB). GTX638748
Anti-Calpain 5 antibody [HL2432] used in IHC (Paraffin sections) (IHC-P). GTX638748
Anti-Calpain 5 antibody [HL2432] used in IHC (Paraffin sections) (IHC-P). GTX638748

Cat. No. GTX638748

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2432

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Calpain 5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human Calpain 5.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

calpain 5 , ADNIV , HTRA3 , VRNI , nCL-3

Background

Calpains are calcium-dependent cysteine proteases involved in signal transduction in a variety of cellular processes. A functional calpain protein consists of an invariant small subunit and 1 of a family of large subunits. CAPN5 is one of the large subunits. Unlike some of the calpains, CAPN5 and CAPN6 lack a calmodulin-like domain IV. Because of the significant similarity to Caenorhabditis elegans sex determination gene tra-3, CAPN5 is also called as HTRA3. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Calpain 5 antibody [HL2432] used in Western Blot (WB). GTX638748

GTX638748 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calpain 5 antibody [HL2432] (GTX638748) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Calpain 5 antibody [HL2432] used in Western Blot (WB). GTX638748

GTX638748 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calpain 5 antibody [HL2432] (GTX638748) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Calpain 5 antibody [HL2432] used in IHC (Paraffin sections) (IHC-P). GTX638748

GTX638748 IHC-P Image

Calpain 5 antibody [HL2432] detects Calpain 5 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
Calpain 5 stained by Calpain 5 antibody [HL2432] (GTX638748) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Calpain 5 antibody [HL2432] used in IHC (Paraffin sections) (IHC-P). GTX638748

GTX638748 IHC-P Image

Calpain 5 antibody [HL2432] detects secreted Calpain 5 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat stomach.
Calpain 5 stained by Calpain 5 antibody [HL2432] (GTX638748) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

There are currently no references for Calpain 5 antibody [HL2432] (GTX638748). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for Calpain 5 antibody [HL2432] (GTX638748). Be the first to share your experience with this product.
Submit a Review
Package List Price ($)
$ 399
$ 169
Custom Conjugations