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Calpain 5 antibody [N1C1]

Anti-Calpain 5 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103264
Anti-Calpain 5 antibody [N1C1] used in Western Blot (WB). GTX103264

Cat No. GTX103264

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3

Predict Reactivity

Mouse, Rat, Bovine, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.39 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Calpain 5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

calpain 5 , ADNIV , HTRA3 , VRNI , nCL-3

Background

Calpains are calcium-dependent cysteine proteases involved in signal transduction in a variety of cellular processes. A functional calpain protein consists of an invariant small subunit and 1 of a family of large subunits. CAPN5 is one of the large subunits. Unlike some of the calpains, CAPN5 and CAPN6 lack a calmodulin-like domain IV. Because of the significant similarity to Caenorhabditis elegans sex determination gene tra-3, CAPN5 is also called as HTRA3. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Calpain 5 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103264

GTX103264 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Calpain-5(GTX103264) antibody at 1:100 dilution.

Anti-Calpain 5 antibody [N1C1] used in Western Blot (WB). GTX103264

GTX103264 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calpain 5 antibody [N1C1] (GTX103264) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169