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Calpain 5 antibody [N1C1]

Orthogonal Overexpression
Anti-Calpain 5 antibody [N1C1] used in Western Blot (WB). GTX103264
Anti-Calpain 5 antibody [N1C1] used in Western Blot (WB). GTX103264
Anti-Calpain 5 antibody [N1C1] used in Western Blot (WB). GTX103264
Anti-Calpain 5 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103264

Cat. No. GTX103264

Reference 5
5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Calpain 5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

Positive Control

mouse uterus , RMS-13

Predict Reactivity

Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.39 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Calpain 5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949829

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

calpain 5 , ADNIV , HTRA3 , VRNI , nCL-3

Background

Calpains are calcium-dependent cysteine proteases involved in signal transduction in a variety of cellular processes. A functional calpain protein consists of an invariant small subunit and 1 of a family of large subunits. CAPN5 is one of the large subunits. Unlike some of the calpains, CAPN5 and CAPN6 lack a calmodulin-like domain IV. Because of the significant similarity to Caenorhabditis elegans sex determination gene tra-3, CAPN5 is also called as HTRA3. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Calpain 5 antibody [N1C1] used in Western Blot (WB). GTX103264

GTX103264 WB Image

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Calpain 5 antibody [N1C1] (GTX103264) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Calpain 5 antibody [N1C1] used in Western Blot (WB). GTX103264

GTX103264 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Calpain 5 antibody [N1C1] (GTX103264) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Calpain 5 antibody [N1C1] used in Western Blot (WB). GTX103264

GTX103264 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calpain 5 antibody [N1C1] (GTX103264) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Calpain 5 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103264

GTX103264 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Calpain-5(GTX103264) antibody at 1:100 dilution.

REFERENCE

Application Reference

JW Geddes et al. Biochim Biophys Acta Mol Basis Dis 2023; Impaired activity and membrane association of most calpain-5 mutants causal for neovascular inflammatory vitreoretinopathy
Application : WB
Vimala Bondada et al. Biochim Biophys Acta Mol Cell Res 2021; 1868 (7) : 119019 The C2 domain of calpain 5 contributes to enzyme activation and membrane localization.
Application : WB
Reactivity : Human
Chukai Y et al. Biochim Biophys Acta Mol Cell Res 2021; 1868 (5) : 118989 Characterization of mitochondrial calpain-5.
Application : WB, ICC/IF
Reactivity : Human
Velez G et al. Cold Spring Harb Mol Case Stud 2018; (Epub) A novel de novo CAPN5 mutation in a patient with inflammatory vitreoretinopathy, hearing loss, and developmental delay.
Application : WB
Schaefer KA et al. Invest Ophthalmol Vis Sci 2016; 57 (6) : 2509-21 Calpain-5 Expression in the Retina Localizes to Photoreceptor Synapses.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

Calpain 5 antibody [N1C1] Cat. No. GTX103264

Rating
( Average 5 based on 5 users reviews)
Application
Western Blot(WB)
( Average 5 based on 5 users reviews)
Anti-Calpain 5 antibody [N1C1] used in Western Blot (WB). GTX103264
Date : Anonymous submitted on 15-Dec-2021
Score :
Application Tested : WB
Sample Species : Ms
Sample : mouse liver tissue lysate
Amount used : 40μg
Blocking : 1% Skim milk in PBST, RT, 1Hr
Primary Antibody : 1:2000, 4°C, 18Hr
Anti-Calpain 5 antibody [N1C1] used in Western Blot (WB). GTX103264
Date : Anonymous submitted on 15-Dec-2021
Score :
Application Tested : WB
Sample Species : Ms
Sample : mouse kidney tissue lysate
Amount used : 40μg
Blocking : 1% Skim milk in PBST, RT, 1Hr
Primary Antibody : 1:2000, 4°C, 18Hr
Anti-Calpain 5 antibody [N1C1] used in Western Blot (WB). GTX103264
Date : Anonymous submitted on 24-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Sample : C57BL/6J mouse liver tissue lysate
Amount used : 40 μg
Blocking : 1% Skim milk in Tw-PBS, 25°C, 1Hr
Primary Antibody : 1:2000, 4°C, 18Hr
Lane Description : Cytosolic calpain 5 of mouse liver.
Anti-Calpain 5 antibody [N1C1] used in Western Blot (WB). GTX103264
Date : Anonymous submitted on 24-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa cells
Amount used : 20 μg
Blocking : 1% Skim milk in Tw-PBS, 25°C, 1Hr
Primary Antibody : 1:2000, 4°C, 18Hr
Lane Description : Mitochondrial calpain 5 of HeLa cells.
Anti-Calpain 5 antibody [N1C1] used in Western Blot (WB). GTX103264
Date : Anonymous submitted on 23-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : HEK293T cells
Amount used : 30 μg
Blocking : 5% skim milk-PBS-0.05% tween20, 25°C, 2Hr
Primary Antibody : 1:1000, 25°C, 1Hr
Lane Description : Lane 1: Transfected with calpain-5 plasmid.
Lane 2: Transfected with empty vector.
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169