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Calpain 5 antibody

Anti-Calpain 5 antibody used in Western Blot (WB). GTX33057
Anti-Calpain 5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33057

Cat. No. GTX33057

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($419)
See all Calpain 5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 361-640 of human CAPN5 (NP_004046.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

calpain 5 , ADNIV , HTRA3 , VRNI , nCL-3

Background

Calpains are calcium-dependent cysteine proteases involved in signal transduction in a variety of cellular processes. A functional calpain protein consists of an invariant small subunit and 1 of a family of large subunits. CAPN5 is one of the large subunits. Unlike some of the calpains, CAPN5 and CAPN6 lack a calmodulin-like domain IV. Because of the significant similarity to Caenorhabditis elegans sex determination gene tra-3, CAPN5 is also called as HTRA3. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Calpain 5 antibody used in Western Blot (WB). GTX33057

GTX33057 WB Image

WB analysis of various sample lysates using GTX33057 Calpain 5 antibody. The signal was developed with ECL plus-Enhanced.
Dilution : 1:1000
Loading : 25μg per lane

Anti-Calpain 5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33057

GTX33057 ICC/IF Image

ICC/IF analysis of NIH/3T3 cells using GTX33057 Calpain 5 antibody.
Blue : DAPI
Dilution : 1:100

REFERENCE

Application Reference

N Morishima et al. J Biol Chem 2024; : 107842 Calpain-5 regulates muscle-specific protein expression and nuclear positioning during myoblast differentiation
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 419