*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
293T , A431 , Jurkat , Raji
Mouse, Rat(>80% identity)
1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.)
Recombinant protein encompassing a sequence within the N-terminus region of human Calpain 9. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
GC36 antibody, nCL-4 antibody, CAPN9 antibody, novel calpain large subunit-4 antibody, new calpain 4 antibody, digestive tract-specific calpain antibody, calpain-9 antibody, calpain 9 antibody
Calpains are ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. The large subunit possesses a cysteine protease domain, and both subunits possess calcium-binding domains. Calpains have been implicated in neurodegenerative processes, as their activation can be triggered by calcium influx and oxidative stress. The protein encoded by this gene is expressed predominantly in stomach and small intestine and may have specialized functions in the digestive tract. This gene is thought to be associated with gastric cancer. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
GTX115472 ICC/IF Image
Calpain 9 antibody [N1N3] detects Calpain 9 protein at cytoplasm by immunofluorescent analysis.
Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Calpain 9 protein stained by Calpain 9 antibody [N1N3] (GTX115472) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
GTX115472 WB Image
Sample (30 ug of whole cell lysate)
7.5% SDS PAGE
GTX115472 diluted at 1:3000