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Calpain S1 antibody

Anti-Calpain S1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118130
Anti-Calpain S1 antibody used in Western Blot (WB). GTX118130
Anti-Calpain S1 antibody used in IHC (Paraffin sections) (IHC-P). GTX118130

Cat. No. GTX118130

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Calpain S1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

293T

Predict Reactivity

Mouse, Rabbit, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Calpain S1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10616854

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

calpain small subunit 1 , CALPAIN4 , CANP , CANPS , CAPN4 , CDPS , CSS1

Cellular Localization

Cytoplasm , Cell membrane

Background

Calpains are a ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. Calpain families have been implicated in neurodegenerative processes, as their activation can be triggered by calcium influx and oxidative stress. Calpain I and II are heterodimeric with distinct large subunits associated with common small subunits, all of which are encoded by different genes. This gene encodes a small subunit common to both calpain I and II and is associated with myotonic dystrophy. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Calpain S1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118130

GTX118130 ICC/IF Image

Calpain S1 antibody detects CAPNS1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: CAPNS1 protein stained by Calpain S1 antibody (GTX118130) diluted at 1:500.
Blue: Hoechst 33343 staining.
Anti-Calpain S1 antibody used in Western Blot (WB). GTX118130

GTX118130 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
12% SDS PAGE
GTX118130 diluted at 1:1000

Anti-Calpain S1 antibody used in IHC (Paraffin sections) (IHC-P). GTX118130

GTX118130 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung cancer, using Calpain S1(GTX118130) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Nowak K et al. Biosci Rep 2018; 38 (5) Inhibition of calpain-1 stabilizes TCF11/Nrf1 but does not affect its activation in response to proteasome inhibition.
Application : WB
Reactivity : Human
Chilla A et al. Mol Cell Proteomics 2013; 12 (7) : 1926-38 Proteomic identification of VEGF-dependent protein enrichment to membrane caveolar-raft microdomains in endothelial progenitor cells.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169