GeneTex
United States (US)

Calpastatin antibody

KO/KD
GTX64549_20180530_WB_18121411_451.jpg
GTX64549_20180822_WB_18121411_668.jpg

Cat No. GTX64549

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
Not tested in other applications.

Calculated MW

77 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 550-650 of human CAST (NP_001177371.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CAST Antibody , BS17 Antibody

Background

The protein encoded by this gene is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The encoded protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jun 2010]

Database

Research Area

DATA IMAGES
GTX64549_20180530_WB_18121411_451.jpg

GTX64549 WB Image

WB analysis of various samples using GTX64549 Calpastatin antibody.
Dilution : 1:1000
Loading : 25μg per lane

GTX64549_20180822_WB_18121411_668.jpg

GTX64549 WB Image

KO/KD

WB analysis of wild type and Calpastatin knockout cell lysate using GTX64549 Calpastatin antibody.
Dilution : 1:1000
Loading : 25μg per lane

Package List Price ($)
$ 319