GeneTex
United States (US)

Calponin-1 antibody [CNN1/832]

GTX34448_20170712_IHC-P_2_18121410_208.jpg
GTX34448_20170712_IHC-P_1_18121410_139.jpg
GTX34448_20170712_IHC-P_18121410_390.jpg

Cat No. GTX34448

Host Mouse
Clonality Monoclonal
Clone Name CNN1/832
Isotype IgG1
Application IHC-P
Reactivity Human, Rat
APPLICATION

Application Note

IHC-P:0.5-1ug/ml for 30 minutes at RT.

Calculated MW

33 kDa. ( Note )

Specificity/Sensitivity

Multiple isoelectric variants of calponin have been identified, however only two molecular weight isoforms exist; a 34kDa form and a 29kDa form. Expression of the 29kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular and visceral smooth muscle. In Western blotting, this MAb reacts with only the 34kDa form of calponin in extracts of human aortic medial smooth muscle and is unreactive with fibroblast extracts of cultivated human foreskin. Calponin is a calmodulin, F-actin and tropomyosin binding protein, which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant human CNN1 protein

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Calponin 1,Hel-S-14,Smcc,Sm-Calp,Cnn1

Database

Research Area

DATA IMAGES
GTX34448_20170712_IHC-P_2_18121410_208.jpg

GTX34448 IHC-P Image

Formalin-fixed, paraffin-embedded Rat Uterus stained with Calponin-1 Monoclonal Antibody (CNN1/832).

GTX34448_20170712_IHC-P_1_18121410_139.jpg

GTX34448 IHC-P Image

Formalin-fixed, paraffin-embedded human Uterus stained with Calponin-1 Monoclonal Antibody (CNN1/832).

GTX34448_20170712_IHC-P_18121410_390.jpg

GTX34448 IHC-P Image

Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Calponin-1 Monoclonal Antibody (CNN1/832).

Package List Price ($)
$ 329