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Calponin 1 antibody [MSVA-455R] HistoMAX™

Recombinant antibody HistoMAX
Anti-Calponin 1 antibody [MSVA-455R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04387
Anti-Calponin 1 antibody [MSVA-455R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04387
Anti-Calponin 1 antibody [MSVA-455R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04387

Cat. No. GTX04387

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 MSVA-455R

Isotype

 IgG

Application

 IHC-P

Reactivity

 Human
Package
500 μl ($629),
100 μl ($299)
See all Calponin 1 products
PRODUCT

Summary

This antibody was validated on 76 different Normal Tissues by IHC-P.

Go to Normal Tissue GalleryGo to Cancer Tissue Gallery

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 1:100-1:200

Note :

IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 9.0 Tris-EDTA-based Target Retrieval Solution buffer. Based on the testing result, the pH 9.0 is optimal but pH 7.8 results in only slightly weaker stainings. At pH 6.0, the immunostaining is markedly reduced.
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

Colon: A moderate to strong Calponin 1 staining should be seen in the muscularis mucosae, muscularis propria and in the walls of larger blood vessels.

Negative Control

Colon: Calponin 1 staining should be absent in all epithelial cells.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA (Please contact us for PBS only format)

Preservative

0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to residues on the C-terminus of human Calponin was used as an immunogen.

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

calponin 1 , HEL-S-14 , SMCC , Sm-Calp

Background

Predicted to enable actin binding activity. Involved in negative regulation of vascular associated smooth muscle cell proliferation. Located in cytoskeleton. [provided by Alliance of Genome Resources, Apr 2022]

Database

Research Area

DATA IMAGES

Anti-Calponin 1 antibody [MSVA-455R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04387

GTX04387 IHC-P Image

IHC-P analysis of human breast tissue using GTX04387 Calponin 1 antibody [MSVA-455R] HistoMAX™.
Calponin 1 immunostaining is regularly seen in myoepithelial cells of the breast.

Anti-Calponin 1 antibody [MSVA-455R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04387

GTX04387 IHC-P Image

IHC-P analysis of human epididymis tissue using GTX04387 Calponin 1 antibody [MSVA-455R] HistoMAX™.
A strong calponin 1 immunostaining ocurrs in the periductal smooth muscle layer in the epididymis.

Anti-Calponin 1 antibody [MSVA-455R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04387

GTX04387 IHC-P Image

IHC-P analysis of human mucosa from descending colon tissue using GTX04387 Calponin 1 antibody [MSVA-455R] HistoMAX™.
A moderate intensity calponin 1 immunostaining is seen in the muscularis mucosae in a colon sample.

REFERENCE

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REVIEW

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Package List Price ($)
$ 629
$ 299