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Calponin 1 antibody, N-term

Anti-Calponin 1 antibody, N-term used in Western Blot (WB). GTX81714
Anti-Calponin 1 antibody, N-term used in Western Blot (WB). GTX81714
Anti-Calponin 1 antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX81714
Anti-Calponin 1 antibody, N-term used in Flow cytometry (FACS). GTX81714

Cat. No. GTX81714

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, FACS

Reactivity

 Human
Package
400 μl ($399)
See all Calponin 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
IHC-P 1:10-1:50
FACS 1:10-1:50
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Sheep, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 10-39 amino acids from the N-terminal region of human Calponin-1.

Purification

Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Conjugation

Unconjugated

RRID

AB_11176394

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

calponin 1 , HEL-S-14 , SMCC , Sm-Calp

Database

Research Area

DATA IMAGES

Anti-Calponin 1 antibody, N-term used in Western Blot (WB). GTX81714

GTX81714 WB Image

WB analysis of HeLa, MDA-MB-453 cell using GTX81714 Calponin-1 antibody, N-term.
Loading : 20ug per lane
Dilution : 1:1000

Anti-Calponin 1 antibody, N-term used in Western Blot (WB). GTX81714

GTX81714 WB Image

WB analysis of NCI-H460 cell lysate (35ug/lane) using GTX81714 Calponin-1 antibody, N-term.

Anti-Calponin 1 antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX81714

GTX81714 IHC-P Image

IHC-P analysis of human lung carcinoma using GTX81714 Calponin-1 antibody, N-term.

Anti-Calponin 1 antibody, N-term used in Flow cytometry (FACS). GTX81714

GTX81714 FACS Image

FACS analysis of NCI-H292 cells using GTX81714 Calponin-1 antibody, N-term.
Top histogram : negative control
Bottom histogram : NCI-H292 cells

REFERENCE

Application Reference

Yang HH et al. Proteomics Clin Appl 2016; (Epub) the putative involvement of actin binding proteins and cytoskeleton proteins in pathological mechanisms of * cystitis - revealed by a prospective pilot study using proteomic approaches.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399