GeneTex
United States (US)

Calponin 2 antibody, Internal

GTX88377_2401_WB_18122102_249.jpg
GTX88377_998_IHC-P_18122102_423.jpg

Cat No. GTX88377

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.03-0.1μg/ml
IHC-P 2.5μg/ml
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88377-PEP).

Specificity/Sensitivity

This antibody is expected to recognize both reported isoforms (NP_004359.1; NP_958434.1), but it is not expected to cross-react with CCN3.
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-DPGEVPEYPPYYQEE, from the internal region (near C Terminus) of the protein sequence according to NP_004359.1; NP_958434.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Calponin 2,Cnn2

Background

The protein encoded by this gene, which can bind actin, calmodulin, troponin C, and tropomyosin, may function in the structural organization of actin filaments. The encoded protein could play a role in smooth muscle contraction and cell adhesion. Several pseudogenes of this gene have been identified, and are present on chromosomes 1, 2, 3, 6, 9, 11, 13, 15, 16, 21 and 22. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2015]

Database

DATA IMAGES
GTX88377_2401_WB_18122102_249.jpg

GTX88377 WB Image

WB analysis of HepG2 lysates using GTX88377 Calponin 2 antibody, Internal.
Dilution : 0.03µg/ml
Loading : 35µg protein in RIPA buffer

GTX88377_998_IHC-P_18122102_423.jpg

GTX88377 IHC-P Image

IHC-P analysis of human placenta using GTX88377 Calponin 2 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 2.5µg/ml

Package List Price ($)
$ 329