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Calponin 3 antibody

Orthogonal
Anti-Calponin 3 antibody used in IHC (Paraffin sections) (IHC-P). GTX106174
Anti-Calponin 3 antibody used in Western Blot (WB). GTX106174
Anti-Calponin 3 antibody used in Western Blot (WB). GTX106174
Anti-Calponin 3 antibody used in Western Blot (WB). GTX106174

Cat No. GTX106174

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)
See all Calponin 3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

293T , HeLa , HepG2 , NIH-3T3 , JC

Predict Reactivity

Bovine, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.86 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Calponin 3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11171002

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

calponin 3

Background

This gene encodes a protein with a markedly acidic C terminus; the basic N-terminus is highly homologous to the N-terminus of a related gene, CNN1. Members of the CNN gene family all contain similar tandemly repeated motifs. This encoded protein is associated with the cytoskeleton but is not involved in contraction. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Calponin 3 antibody used in IHC (Paraffin sections) (IHC-P). GTX106174

GTX106174 IHC-P Image

calponin 3 antibody detects calponin 3 protein at cytoplasm in rat ovary by immunohistochemical analysis.
Sample: Paraffin-embedded rat ovary.
calponin 3 antibody (GTX106174) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Calponin 3 antibody used in Western Blot (WB). GTX106174

GTX106174 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: HeLa
C: HepG2
10% SDS PAGE
GTX106174 diluted at 1:5000

Anti-Calponin 3 antibody used in Western Blot (WB). GTX106174

GTX106174 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
10% SDS PAGE
GTX106174 diluted at 1:1000

Anti-Calponin 3 antibody used in Western Blot (WB). GTX106174

GTX106174 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Calponin 3 antibody (GTX106174) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Paredes F et al. Circ Res 2019; (Epub) Mitochondrial Protein Poldip2 Controls VSMC Differentiated Phenotype by O-Linked GlcNAc Transferase-Dependent Inhibition of a Ubiquitin Proteasome System.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169