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Calponin 3 antibody

KO/KD Orthogonal
Anti-Calponin 3 antibody used in Western Blot (WB). GTX106174
Anti-Calponin 3 antibody used in Western Blot (WB). GTX106174
Anti-Calponin 3 antibody used in IHC (Paraffin sections) (IHC-P). GTX106174
Anti-Calponin 3 antibody used in Western Blot (WB). GTX106174

Cat. No. GTX106174

Reference 1
3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Calponin 3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

293T , HeLa , HepG2 , NIH-3T3 , JC

Predict Reactivity

Bovine, Chicken, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.86 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Calponin 3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11171002

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

calponin 3

Background

This gene encodes a protein with a markedly acidic C terminus; the basic N-terminus is highly homologous to the N-terminus of a related gene, CNN1. Members of the CNN gene family all contain similar tandemly repeated motifs. This encoded protein is associated with the cytoskeleton but is not involved in contraction. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Calponin 3 antibody used in Western Blot (WB). GTX106174

GTX106174 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: HeLa
C: HepG2
10% SDS PAGE
GTX106174 diluted at 1:5000

Anti-Calponin 3 antibody used in Western Blot (WB). GTX106174

GTX106174 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
10% SDS PAGE
GTX106174 diluted at 1:1000

Anti-Calponin 3 antibody used in IHC (Paraffin sections) (IHC-P). GTX106174

GTX106174 IHC-P Image

calponin 3 antibody detects calponin 3 protein at cytoplasm in rat ovary by immunohistochemical analysis.
Sample: Paraffin-embedded rat ovary.
calponin 3 antibody (GTX106174) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Calponin 3 antibody used in Western Blot (WB). GTX106174

GTX106174 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Calponin 3 antibody (GTX106174) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Paredes F et al. Circ Res 2019; (Epub) Mitochondrial Protein Poldip2 Controls VSMC Differentiated Phenotype by O-Linked GlcNAc Transferase-Dependent Inhibition of a Ubiquitin Proteasome System.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

Calponin 3 antibody Cat. No. GTX106174

Rating
( Average 5 based on 3 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
Immunoprecipitation(IP)
( Average 5 based on 1 users reviews)
Anti-Calponin 3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106174
Date : Anonymous submitted on 13-Sep-2023
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : HAP1 WT and CNN3 KO cells
Blocking : PBS, 5% BSA, 5% goat serum, 0.01% Triton X-100, 25°C, 0.5Hr
Permeabilization /Antigen retrieval : PBS, 0.1% Triton X-100, 25°C, 0.17Hr
Primary Antibody : 1:800, 4°C, 16Hr
Figure Note : WT cells: Green dashed line KO cells: Magenta dashed line Data provided by YCharOS Inc., an open science company, the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Anti-Calponin 3 antibody used in Immunoprecipitation (IP). GTX106174
Date : Anonymous submitted on 13-Sep-2023
Score :
Application Tested : IP
Sample Species : Hu
Sample : HAP1 cells
Lane Description : SM: 4% starting material UB: 4% unbound fraction HC: antibody heavy chain
Figure Note : Antibody-bead conjugates were prepared by adding 2.0 μg of antibody to 500 μL of IP Lysis Buffer, together, 30 μL of protein A-Sepharose beads. 0.5 mL aliquots at 2.0 mg/mL of lysate were incubated, an antibody-bead conjugate for ~1 hrs at 4ºC. Data provided by YCharOS Inc., an open science company, the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Anti-Calponin 3 antibody used in Western Blot (WB). GTX106174
Date : Anonymous submitted on 13-Sep-2023
Score :
Application Tested : WB
Sample Species : Hu
Sample : HAP1 WT and CNN3 KO cells
Amount used : 20 μg
Primary Antibody : 1:1000, 4°C, 16Hr
Figure Note : Data provided by YCharOS Inc., an open science company, the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169