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Calponin 3 antibody

Anti-Calponin 3 antibody used in Western Blot (WB). GTX118017

Cat. No. GTX118017

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IP

Reactivity

Human
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IP Assay dependent
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

293T , HeLa , HepG2

Predict Reactivity

Mouse, Rat, Bovine, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Calponin 3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885433

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

calponin 3

Background

This gene encodes a protein with a markedly acidic C terminus; the basic N-terminus is highly homologous to the N-terminus of a related gene, CNN1. Members of the CNN gene family all contain similar tandemly repeated motifs. This encoded protein is associated with the cytoskeleton but is not involved in contraction. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Calponin 3 antibody used in Western Blot (WB). GTX118017

GTX118017 WB Image

calponin 3 antibody detects calponin 3 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg HeLa whole cell lysate/extract
C. 30 μg HepG2 whole cell lysate/extract
10 % SDS-PAGE
calponin 3 antibody (GTX118017) dilution: 1:1000

REFERENCE

There are currently no references for Calponin 3 antibody (GTX118017). Be the first to share your publications with this product.

REVIEW

Calponin 3 antibody Cat. No. GTX118017

Rating ( Average 5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Immunoprecipitation(IP) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 13-Sep-2023
Score :
Application Tested : IP
Sample Species : Hu
Sample : HAP1 cells
Lane Description : SM: 4% starting material UB: 4% unbound fraction HC: antibody heavy chain
Figure Note : Antibody-bead conjugates were prepared by adding 2.0 μg of antibody to 500 μL of IP Lysis Buffer, together, 30 μL of protein A-Sepharose beads. 0.5 mL aliquots at 2.0 mg/mL of lysate were incubated, an antibody-bead conjugate for ~1 hrs at 4ºC. Data provided by YCharOS Inc., an open science company, the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Date : Anonymous submitted on 13-Sep-2023
Score :
Application Tested : WB
Sample Species : Hu
Sample : HAP1 WT and CNN3 KO cells
Amount used : 20 μg
Primary Antibody : 1:1000, 4°C, 16Hr
Figure Note : Data provided by YCharOS Inc., an open science company, the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169