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Calreticulin 3 antibody

Anti-Calreticulin 3 antibody used in Western Blot (WB). GTX00741
Anti-Calreticulin 3 antibody used in IHC (Frozen sections) (IHC-Fr). GTX00741
Anti-Calreticulin 3 antibody used in Western Blot (WB). GTX00741
Anti-Calreticulin 3 antibody used in Immunoprecipitation (IP). GTX00741
Anti-Calreticulin 3 antibody used in Western Blot (WB). GTX00741

Cat No. GTX00741

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-Fr, IP

Reactivity

Mouse
Package
100 μl ($359)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000
IHC-Fr 1:100
IP 1:100
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Serum with 0.1% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Mouse

Immunogen

Synthetic peptide corresponding to C-terminal region of mouse CALR3, CMGKFHRHNHLSRFHRQGEL.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

calreticulin 3 , 1700031L01Rik , 6330586I20Rik , Crt2 , cspn

Database

Research Area

DATA IMAGES

Anti-Calreticulin 3 antibody used in Western Blot (WB). GTX00741

GTX00741 WB Image

WB analysis of embryonic fibroblast cells prepared from Calr3 -/- mouse using GTX00741 Calreticulin 3 antibody.
Lane 1 : Mock
Lane 2 : Cells overexpressed Calr3 protein
Dilution : 1:1000

Anti-Calreticulin 3 antibody used in IHC (Frozen sections) (IHC-Fr). GTX00741

GTX00741 IHC-Fr Image

IHC-Fr analysis of mouse testicular tissue section (8 μm) using GTX00741 Calreticulin 3 antibody.
Upper : hematoxylin and eosin staining
Lower : Primary antibody
Dilution : 1:100
Fixation : 4% PFA in PBS, overnight, 4 degree C
Scale bar : 200 μm

Anti-Calreticulin 3 antibody used in Western Blot (WB). GTX00741

GTX00741 WB Image

WB analysis of mouse testis or sperm tissue lysate using GTX00741 Calreticulin 3 antibody. This protein is testis specific and absent in mature sperm.
Dilution : 1:1000
Loading : 10 μg
SDS-PAGE : 10-20% gradient gel
Specifically expressed during male meiotic germ cell development. Not detectable in mature spermatids (Uniprot#P52194).

Anti-Calreticulin 3 antibody used in Immunoprecipitation (IP). GTX00741

GTX00741 IP Image

IP analysis of wild-type mouse testis using GTX00741 Calreticulin 3 antibody.
Lane 1 : Input mouse testis lysate
Lane 2 : Precipitated with preimmune serum
Lane 3 : Precipitated with anti-CALR antibody

Anti-Calreticulin 3 antibody used in Western Blot (WB). GTX00741

GTX00741 WB Image

WB analysis of various mouse tissue samples using GTX00741 Calreticulin 3 antibody.
Lane 1 : mouse brain tissue lysate
Lane 2 : mouse lung tissue lysate
Lane 3 : mouse heart tissue lysate
Lane 4 : mouse thymus tissue lysate
Lane 5 : mouse liver tissue lysate
Lane 6 : mouse spleen tissue lysate
Lane 7 : mouse kidney tissue lysate
Lane 8 : mouse muscle tissue lysate
Lane 9 : mouse ovary tissue lysate
Lane 10 : mouse testis tissue lysate
Lane 11 : mouse sperm tissue lysate
Dilution : 1:1000
This protein is testis specific and absent in mature sperm (Uniprot#Q9D9Q6).

REFERENCE

There are currently no references for Calreticulin 3 antibody (GTX00741). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Calreticulin 3 antibody (GTX00741). Be the first to share your experience with this product.
SDS
Sodium Azide.pdf