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Calreticulin 3 antibody

Orthogonal Overexpression
Anti-Calreticulin 3 antibody used in Immunoprecipitation (IP). GTX00741
Anti-Calreticulin 3 antibody used in Western Blot (WB). GTX00741
Anti-Calreticulin 3 antibody used in IHC (Frozen sections) (IHC-Fr). GTX00741
Anti-Calreticulin 3 antibody used in Western Blot (WB). GTX00741
Anti-Calreticulin 3 antibody used in Western Blot (WB). GTX00741

Cat. No. GTX00741

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-Fr, IP

Reactivity

 Mouse
Package
100 μl ($399)
See all Calreticulin 3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
IHC-Fr 1:100
IP 1:100
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Serum

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Mouse

Immunogen

Synthetic peptide corresponding to C-terminal region of mouse CALR3, CMGKFHRHNHLSRFHRQGEL.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

calreticulin 3 , 1700031L01Rik , 6330586I20Rik , Crt2 , cspn

Database

Research Area

DATA IMAGES

Anti-Calreticulin 3 antibody used in Immunoprecipitation (IP). GTX00741

GTX00741 IP Image

IP analysis of wild-type mouse testis using GTX00741 Calreticulin 3 antibody.
Lane 1 : Input mouse testis lysate
Lane 2 : Precipitated with preimmune serum
Lane 3 : Precipitated with anti-CALR antibody

Anti-Calreticulin 3 antibody used in Western Blot (WB). GTX00741

GTX00741 WB Image

Overexpression

WB analysis of embryonic fibroblast cells prepared from Calr3 -/- mouse using GTX00741 Calreticulin 3 antibody.
Lane 1 : Mock
Lane 2 : Cells overexpressed Calr3 protein
Dilution : 1:1000

Anti-Calreticulin 3 antibody used in IHC (Frozen sections) (IHC-Fr). GTX00741

GTX00741 IHC-Fr Image

IHC-Fr analysis of mouse testicular tissue section (8 μm) using GTX00741 Calreticulin 3 antibody.
Upper : hematoxylin and eosin staining
Lower : Primary antibody
Dilution : 1:100
Fixation : 4% PFA in PBS, overnight, 4 degree C
Scale bar : 200 μm

Anti-Calreticulin 3 antibody used in Western Blot (WB). GTX00741

GTX00741 WB Image

Orthogonal

WB analysis of mouse testis or sperm tissue lysate using GTX00741 Calreticulin 3 antibody. This protein is testis specific and absent in mature sperm.
Dilution : 1:1000
Loading : 10 μg
SDS-PAGE : 10-20% gradient gel
Specifically expressed during male meiotic germ cell development. Not detectable in mature spermatids (Uniprot#P52194).

Anti-Calreticulin 3 antibody used in Western Blot (WB). GTX00741

GTX00741 WB Image

Orthogonal

WB analysis of various mouse tissue samples using GTX00741 Calreticulin 3 antibody.
Lane 1 : mouse brain tissue lysate
Lane 2 : mouse lung tissue lysate
Lane 3 : mouse heart tissue lysate
Lane 4 : mouse thymus tissue lysate
Lane 5 : mouse liver tissue lysate
Lane 6 : mouse spleen tissue lysate
Lane 7 : mouse kidney tissue lysate
Lane 8 : mouse muscle tissue lysate
Lane 9 : mouse ovary tissue lysate
Lane 10 : mouse testis tissue lysate
Lane 11 : mouse sperm tissue lysate
Dilution : 1:1000
This protein is testis specific and absent in mature sperm (Uniprot#Q9D9Q6).

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399