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Calreticulin 3 antibody

Anti-Calreticulin 3 antibody used in IHC (Paraffin sections) (IHC-P). GTX112177
Anti-Calreticulin 3 antibody used in Western Blot (WB). GTX112177
Anti-Calreticulin 3 antibody used in IHC (Paraffin sections) (IHC-P). GTX112177

Cat. No. GTX112177

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Calreticulin 3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

Molt-4 , Raji

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Calreticulin 3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949800

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

calreticulin 3 , CMH19 , CRT2 , CT93

Cellular Localization

Endoplasmic reticulum lumen

Background

Calreticulins, such as CALR3, are Ca(2+)-binding chaperones localized mainly in the endoplasmic/sarcoplasmic reticulum Persson et al. (2002) [PubMed 12384296].[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Calreticulin 3 antibody used in IHC (Paraffin sections) (IHC-P). GTX112177

GTX112177 IHC-P Image

Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using Calreticulin 3(GTX112177) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Calreticulin 3 antibody used in Western Blot (WB). GTX112177

GTX112177 WB Image

Sample (30 ug of whole cell lysate)
A: Molt-4 (GTX27912)
B: Raji
10% SDS PAGE
GTX112177 diluted at 1:1000

Anti-Calreticulin 3 antibody used in IHC (Paraffin sections) (IHC-P). GTX112177

GTX112177 IHC-P Image

Calreticulin 3 antibody detects Calreticulin 3 protein at cytoplasm in mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
Calreticulin 3 antibody (GTX112177) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169