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Calreticulin antibody

Overexpression
Anti-Calreticulin antibody used in Immunoprecipitation (IP). GTX00740
Anti-Calreticulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00740
Anti-Calreticulin antibody used in Western Blot (WB). GTX00740
Anti-Calreticulin antibody used in Western Blot (WB). GTX00740
Anti-Calreticulin antibody used in IHC (Paraffin sections) (IHC-P). GTX00740
Anti-Calreticulin antibody used in Western Blot (WB). GTX00740

Cat. No. GTX00740

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all Calreticulin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:300-1:1000
IHC-P 1:1000
IP 1:100
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Serum

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Mouse

Immunogen

C-EEDEKEEDEEESPGQAKDEL (C-terminal of mouse CALR protein)

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

calreticulin , CRT , Calregulin

Database

Research Area

DATA IMAGES

Anti-Calreticulin antibody used in Immunoprecipitation (IP). GTX00740

GTX00740 IP Image

IP analysis of wild-type mouse testis samples using GTX00740 Calreticulin antibody.
Lane 1 : Input mouse testis lysate
Lane 2 : Precipitated with preimmune serum
Lane 3 : Precipitated with anti-CALR antibody

Anti-Calreticulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00740

GTX00740 ICC/IF Image

ICC/IF analysis of NIH-3T3 cells using GTX00740 Calreticulin antibody.
Dilution : 1:300
Fixation : 4% PFA
Permeabilization : 0.5% Triton X-100

Anti-Calreticulin antibody used in Western Blot (WB). GTX00740

GTX00740 WB Image

WB analysis of various samples using GTX00740 Calreticulin antibody.
Lane 1 : HeLa whole cell lysate
Lane 2 : MCF-7 whole cell lysate
Dilution : 1:1000
Loading : 10 μg
SDS-PAGE : 12.5%

Anti-Calreticulin antibody used in Western Blot (WB). GTX00740

GTX00740 WB Image

Overexpression

WB analysis of embryonic fibroblast cells prepared from Calr -/- mouse using GTX00740 Calreticulin antibody.
Lane 1 : Mock
Lane 2 : Cells overexpressed Calr protein
Dilution : 1:500

Anti-Calreticulin antibody used in IHC (Paraffin sections) (IHC-P). GTX00740

GTX00740 IHC-P Image

IHC-P analysis of mouse testis tissue section using GTX00740 Calreticulin antibody.
Dilution : 1:1000

Anti-Calreticulin antibody used in Western Blot (WB). GTX00740

GTX00740 WB Image

WB analysis of mouse testis and sperm using GTX00740 Calreticulin antibody.
Dilution : 1:1000
Loading : 10 μg
SDS-PAGE : 10-20% gradient gel

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399