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Calreticulin antibody

Anti-Calreticulin antibody used in IHC (Paraffin sections) (IHC-P). GTX111627
Anti-Calreticulin antibody used in Western Blot (WB). GTX111627
Anti-Calreticulin antibody used in Western Blot (WB). GTX111627
Anti-Calreticulin antibody used in IHC (Paraffin sections) (IHC-P). GTX111627
Anti-Calreticulin antibody used in Western Blot (WB). GTX111627
Anti-Calreticulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111627

Cat No. GTX111627

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse, Rat, Bovine
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , A375 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2

Product Note

IP/MS validation was supported by references (PMID:30377405)

Predict Reactivity

Zebrafish, Rabbit, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.38 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Calreticulin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11175861

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

calreticulin , CRT , HEL-S-99n , RO , SSA , cC1qR

Cellular Localization

Endoplasmic reticulum lumen , Cytoplasm , cytosol , Secreted , extracellular space , extracellular matrix , Cell surface

Background

Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin can inhibit the binding of androgen receptor to its hormone-responsive DNA element and can inhibit androgen receptor and retinoic acid receptor transcriptional activities in vivo, as well as retinoic acid-induced neuronal differentiation. Thus, calreticulin can act as an important modulator of the regulation of gene transcription by nuclear hormone receptors. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin but calreticulin is not a Ro/SS-A antigen. Earlier papers referred to calreticulin as an Ro/SS-A antigen but this was later disproven. Increased autoantibody titer against human calreticulin is found in infants with complete congenital heart block of both the IgG and IgM classes. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Calreticulin antibody used in IHC (Paraffin sections) (IHC-P). GTX111627

GTX111627 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using Calreticulin(GTX111627) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Calreticulin antibody used in Western Blot (WB). GTX111627

GTX111627 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Calreticulin antibody (GTX111627) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Calreticulin antibody used in Western Blot (WB). GTX111627

GTX111627 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Calreticulin antibody (GTX111627) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Calreticulin antibody used in IHC (Paraffin sections) (IHC-P). GTX111627

GTX111627 IHC-P Image

Immunohistochemical analysis of paraffin-embedded mouse muscle, using Calreticulin(GTX111627) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Calreticulin antibody used in Western Blot (WB). GTX111627

GTX111627 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Calreticulin antibody (GTX111627) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Calreticulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111627

GTX111627 ICC/IF Image

Calreticulin antibody detects Calreticulin protein at endoplasmic reticulum by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Calreticulin stained by Calreticulin antibody (GTX111627) diluted at 1:500.

REFERENCE

REVIEW

Calreticulin antibody Cat No. GTX111627

Rating ( Average 5 based on 3 users reviews)
Application
Western Blot(WB) ( Average 5 based on 3 users reviews)
Date : Anonymous submitted on 05-May-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells
Amount used : 10 μg
Blocking : 5% skim milk in TBST, 27°C, 1Hr
Primary Antibody : 1:5000, 4°C, 12Hr
Secondary Antibody : GTX213110-01, 1:5000, 27°C, 2Hr
Lane Description : Two groups (Dengue Nucleocapsid protein -/+) of whole cell extracts (10 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Calreticulin antibody (GTX111627) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Bov
Figure Note : The data was peer reviewed and published in the journal Dis Model Mech in 2016.PMID: 27483354
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017.PMID: 28216671
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169