GeneTex
United States (US)

Calreticulin antibody

Calreticulin-antibody_WB_GTX16144-1_18121409_875.jpg

Cat No. GTX16144

Host Sheep
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse, Rat, Dog
APPLICATION

Application Note

For IF: Use at a dilution of 1:50. For WB: Use at a dilution of 1:1,000 - 1:5,000. Predicted molecular weight: 48 kDa. Could be used as a marker for subcellular fractionation of cells. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

48 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

Phosphate-buffered saline, pH 7.2, containing 0.08% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

C-terminal domain of human calreticulin

Purification

IgG fraction
From polyclonal serum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Calreticulin,Crt,Hel-S-99N,Ro,Ssa,Cc1Qr,Calr

Cellular Localization

Endoplasmic reticulum lumen. Also found in cell surface (T cells), cytosol and extracellular matrix. Associated with the lytic granules in the cytolytic T-lymphocytes.

Background

Calreticulin is an abundant highly conserved calcium binding protein found in the lumen of the endoplasmic reticulum of eukaryotic cells. This lumen protein can be used to measure the intactness of membrane preparations.

Database

DATA IMAGES
Calreticulin-antibody_WB_GTX16144-1_18121409_875.jpg

GTX16144 WB Image

Western blot using antibody at 1:5000 dilution on indicated samples. Immunofluorescence of MCF-7 cells stained with antibody at 1:50 dilution.

Package List Price ($)
$ 389