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Calreticulin antibody

Anti-Calreticulin antibody used in IHC (Paraffin sections) (IHC-P). GTX20004
Anti-Calreticulin antibody used in IHC (Paraffin sections) (IHC-P). GTX20004
Anti-Calreticulin antibody used in Western Blot (WB). GTX20004
Anti-Calreticulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX20004
Anti-Calreticulin antibody used in Western Blot (WB). GTX20004

Cat. No. GTX20004

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, Dot, EM, Neutralizing/Inhibition

Reactivity

 Human, Mouse, Rat, Bovine, Hamster, Primate
Package
100 μl ($419)
See all Calreticulin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:50 - 1:250
IHC-P 1:50 -1:200
IP Assay dependent
Dot Assay dependent
EM Assay dependent
Neutralizing/Inhibition Assay dependent
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

A fusion protein to mouse Calreticulin [UniProt# P14211]

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_368200

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

calreticulin , CRT , Calregulin

Background

Molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export.

Database

Research Area

DATA IMAGES

Anti-Calreticulin antibody used in IHC (Paraffin sections) (IHC-P). GTX20004

GTX20004 IHC-P Image

IHC-P analysis of human thyroid gland tissue using GTX20004 Calreticulin antibody.
Dilution : 1:50

Anti-Calreticulin antibody used in IHC (Paraffin sections) (IHC-P). GTX20004

GTX20004 IHC-P Image

IHC-P analysis of human thyroid gland tissue using GTX20004 Calreticulin antibody.
Dilution : 1:50

Anti-Calreticulin antibody used in Western Blot (WB). GTX20004

GTX20004 WB Image

WB analysis of HeLa cell lysate using GTX20004 Calreticulin antibody.

Anti-Calreticulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX20004

GTX20004 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX20004 Calreticulin antibody.
Green : primary antibody
Red : Tubulin
Blue : DAPI
Dilution : 1:250

Anti-Calreticulin antibody used in Western Blot (WB). GTX20004

GTX20004 WB Image

WB analysis of human kidney tissue lysate using GTX20004 Calreticulin antibody.

REFERENCE

Application Reference

Xu C et al. J Biol Chem 2008; 283 (17) : 11477-84 BI-1 regulates endoplasmic reticulum Ca2+ homeostasis downstream of Bcl-2 family proteins.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 419