GeneTex
United States (US)

Calretinin antibody

Overexpression
GTX103261_39876_WB_M_Brain_19011506_523.jpg
GTX103261_39876_20160602_IFA_19011506_728.jpg
GTX103261_39876_20160808_IHC-Fr_19011506_236.jpg
GTX103261_39876_CT_IHC_M_19011506_818.jpg
GTX103261_39876_IHC_M_19011506_119.jpg
GTX103261_39876_20171017_IHC-Fr_M_19011506_566.jpg
GTX103261_39876_WB_B_19011506_870.jpg
GTX103261_WB_19011506_829.jpg
GTX103261_39876_20160414_WB_R_brain_19011506_169.jpg

Cat No. GTX103261

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Positive Control

A431 , human CALB2-transfected 293T cells , mouse brain, rat brain

Predict Reactivity

Bovine, Chicken, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.75mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Calretinin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CAB29 antibody, CAL2 antibody, CR antibody, CALB2 antibody, calbindin 2, (29kD, calretinin) antibody, 29 kDa calbindin antibody, calbindin D29K antibody, calretinin antibody, calbindin 2 antibody

Background

This gene encodes an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. Three alternatively spliced transcript variants that encode different proteins have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX103261_39876_WB_M_Brain_19011506_523.jpg

GTX103261 WB Image

Sample (50 µg of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX103261 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX103261_39876_20160602_IFA_19011506_728.jpg

GTX103261 ICC/IF Image

Calretinin antibody detects Calretinin protein at cytoplasm by immunofluorescent analysis.
Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Calretinin protein stained by Calretinin antibody (GTX103261) diluted at 1:200.
Blue: Hoechst 33342 staining.

GTX103261_39876_20160808_IHC-Fr_19011506_236.jpg

GTX103261 IHC-Fr Image

Calretinin antibody detects Calretinin protein in the amacrine cells by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: Calretinin protein stained by Calretinin antibody (GTX103261) diluted at 1:250.
Red: Protein kinase C alpha staining.
Blue: Fluoroshield with DAPI (GTX30920).

GTX103261_39876_CT_IHC_M_19011506_818.jpg

GTX103261 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Mouse retina, using Calretinin(GTX103261) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX103261_39876_IHC_M_19011506_119.jpg

GTX103261 IHC-P Image

Calretinin antibody detects Calretinin protein at cytosol on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
Calretinin antibody (GTX103261) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX103261_39876_20171017_IHC-Fr_M_19011506_566.jpg

GTX103261 IHC-Fr Image

Calretinin antibody detects Calretinin protein by immunohistochemical analysis.
Samples: Frozen Section adult mouse hippocampus.
Green: Calretinin protein stained by Calretinin antibody (GTX103261) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX103261_39876_WB_B_19011506_870.jpg

GTX103261 WB Image

Overexpression

Calretinin antibody detects CALB2 protein by western blot analysis.
A. 30 µg 293T whole cell lysate/extract
B. 30 µg whole cell lysate/extract of human CALB2-transfected 293T cells
12% SDS-PAGE
Calretinin antibody (GTX103261) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX103261_WB_19011506_829.jpg

GTX103261 WB Image

Sample (30 µg of whole cell lysate)
A: A431 (GTX27909)
12% SDS PAGE
Calretinin antibody
GTX103261 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX103261_39876_20160414_WB_R_brain_19011506_169.jpg

GTX103261 WB Image

Rat tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with Calretinin antibody (GTX103261) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

Calretinin antibody Cat No. GTX103261

Rating ( Average 3 based on 1 users reviews)
Application
IHC (Paraffin sections)(IHC-P) ( Average 3 based on 1 users reviews)
Date : Anonymous submitted on 20-Jan-2013
Score :
Application Tested : IHC-P
Sample Species : Ms
Sample : Mouse retina
Blocking : 10% donkey serum, 25°C, 1Hr
Fixation : 4% paraformaldehyde
Permeabilization /Antigen retrieval : 0.5% Trition X-100
Primary Antibody : 1:250, 4°C, 48Hr
Figure Note : Green channel:calretinin(GTX103261), Red channel:Cx36, Scale bar=50μm
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169