GeneTex
  • Country / Location Selection

United States (US)

Calretinin antibody

Anti-Calretinin antibody used in Western Blot (WB). GTX103261
Anti-Calretinin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103261
Anti-Calretinin antibody used in IHC (Frozen sections) (IHC-Fr). GTX103261
Anti-Calretinin antibody used in IHC (Paraffin sections) (IHC-P). GTX103261
Anti-Calretinin antibody used in IHC (Paraffin sections) (IHC-P). GTX103261
Anti-Calretinin antibody used in IHC (Frozen sections) (IHC-Fr). GTX103261
Anti-Calretinin antibody used in Western Blot (WB). GTX103261
Anti-Calretinin antibody used in Western Blot (WB). GTX103261
Anti-Calretinin antibody used in Western Blot (WB). GTX103261

Cat No. GTX103261

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Positive Control

A431 , human CALB2-transfected 293T cells , mouse brain, rat brain

Predict Reactivity

Bovine, Chicken, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.75 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Calretinin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

calbindin 2 , CAB29 , CAL2 , CR

Background

This gene encodes an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. Three alternatively spliced transcript variants that encode different proteins have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Calretinin antibody used in Western Blot (WB). GTX103261

GTX103261 WB Image

Sample (50 µg of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX103261 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Calretinin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103261

GTX103261 ICC/IF Image

Calretinin antibody detects Calretinin protein at cytoplasm by immunofluorescent analysis.
Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Calretinin protein stained by Calretinin antibody (GTX103261) diluted at 1:200.
Blue: Hoechst 33342 staining.

Anti-Calretinin antibody used in IHC (Frozen sections) (IHC-Fr). GTX103261

GTX103261 IHC-Fr Image

Calretinin antibody detects Calretinin protein in the amacrine cells by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: Calretinin protein stained by Calretinin antibody (GTX103261) diluted at 1:250.
Red: Protein kinase C alpha staining.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Calretinin antibody used in IHC (Paraffin sections) (IHC-P). GTX103261

GTX103261 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Mouse retina, using Calretinin(GTX103261) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Calretinin antibody used in IHC (Paraffin sections) (IHC-P). GTX103261

GTX103261 IHC-P Image

Calretinin antibody detects Calretinin protein at cytosol on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
Calretinin antibody (GTX103261) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Calretinin antibody used in IHC (Frozen sections) (IHC-Fr). GTX103261

GTX103261 IHC-Fr Image

Calretinin antibody detects Calretinin protein by immunohistochemical analysis.
Samples: Frozen Section adult mouse hippocampus.
Green: Calretinin protein stained by Calretinin antibody (GTX103261) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Calretinin antibody used in Western Blot (WB). GTX103261

GTX103261 WB Image

Calretinin antibody detects CALB2 protein by western blot analysis.
A. 30 µg 293T whole cell lysate/extract
B. 30 µg whole cell lysate/extract of human CALB2-transfected 293T cells
12% SDS-PAGE
Calretinin antibody (GTX103261) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Calretinin antibody used in Western Blot (WB). GTX103261

GTX103261 WB Image

Sample (30 µg of whole cell lysate)
A: A431 (GTX27909)
12% SDS PAGE
Calretinin antibody
GTX103261 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Calretinin antibody used in Western Blot (WB). GTX103261

GTX103261 WB Image

Rat tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with Calretinin antibody (GTX103261) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

Calretinin antibody Cat No. GTX103261

Rating ( Average 3 based on 1 users reviews)
Application
IHC (Paraffin sections)(IHC-P) ( Average 3 based on 1 users reviews)
Date : Anonymous submitted on 20-Jan-2013
Score :
Application Tested : IHC-P
Sample Species : Ms
Sample : Mouse retina
Blocking : 10% donkey serum, 25°C, 1Hr
Fixation : 4% paraformaldehyde
Permeabilization /Antigen retrieval : 0.5% Trition X-100
Primary Antibody : 1:250, 4°C, 48Hr
Figure Note : Green channel:calretinin(GTX103261), Red channel:Cx36, Scale bar=50μm
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169