IP: Use at a dilution of 1/250. WB: Use at a dilution of 1/1000. Detects a band of approximately 29 kDa. Optimal dilutions/concentrations should be determined by the end user.
Rat brain homogenates.
Has no affinity for other calcium binding proteins including Calbindin, parvalbumin, and S100 protein.
Preservative: 0.02% Thimerosal / Merthiolate
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Synthetic cyclic peptide (common to all Calretinin proteins) (Human).
Immunogen affinity purified
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Calretinin is a 29 kDa calcium binding protein that is expressed in central and peripheral nervous system and in many normal and pathological tissues. Calretinin is also considered an important diagnostic aid in the differential diagnosis of cystic and solid ameloblastic tumors. The rat and human calretinin exhibit 98% sequence homology and 91% homology to many other species. Calretinin is generally expressed with two other calcium binding proteins Calbindin D-29 and parvalbumin in CNS and other peripheral tissues. Two calcium-binding proteins, calbindin and calretinin, have been reported to be expressed in abundance in Purkinje cells and other cell types in the cerebellum. The antibodies against calretinin also labels the mossy fibers throughout, and climbing fibers in a distinct group of folia. They also stain several cell types in the molecular layer. There is no appreciable immunostaining of calretinin in cerebellar cortex. In the rat, antibodies against calretinin stain the Lugaro cells, and some granule cells in lobe X; they also give weak staining of all the granule cells in the other lobes. Calretinin antibodies have been used to detect a number of carcinomas by clinicopathological, immunohistochemical techniques including, epithelial malignant mesotheliomas, Ovarian yolk sac tumor, Adenomatoid tumors of the uterus etc.
GTX14689 WB Image
Western blot using anti-Calretinin antibody (GTX14689) & calretinin WB positive control.
GTX14689 IHC-P Image
IHC-P analysis of rat brain using GTX14689 at a dilution of 1:100.