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Calsequestrin 2 antibody

Anti-Calsequestrin 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX105914
Anti-Calsequestrin 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX105914
Anti-Calsequestrin 2 antibody used in Western Blot (WB). GTX105914

Cat. No. GTX105914

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Calsequestrin 2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

HCT116

Predict Reactivity

Rat, Rabbit, Bovine, Dog, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.68 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Calsequestrin 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949842

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

calsequestrin 2 , PDIB2

Cellular Localization

Sarcoplasmic reticulum lumen

Background

The protein encoded by this gene specifies the cardiac muscle family member of the calsequestrin family. Calsequestrin is localized to the sarcoplasmic reticulum in cardiac and slow skeletal muscle cells. The protein is a calcium binding protein that stores calcium for muscle function. Mutations in this gene cause stress-induced polymorphic ventricular tachycardia, also referred to as catecholaminergic polymorphic ventricular tachycardia 2 (CPVT2), a disease characterized by bidirectional ventricular tachycardia that may lead to cardiac arrest. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Calsequestrin 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX105914

GTX105914 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft , using Calsequestrin-2(GTX105914) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Calsequestrin 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX105914

GTX105914 IHC-P Image

Calsequestrin-2 antibody detects Calsequestrin-2 protein at cytosol on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
Calsequestrin-2 antibody (GTX105914) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Calsequestrin 2 antibody used in Western Blot (WB). GTX105914

GTX105914 WB Image

Calsequestrin 2 antibody detects Calsequestrin 2 protein by western blot analysis.
A. 30 μg HCT116 whole cell lysate/extract
10 % SDS-PAGE
Calsequestrin 2 antibody (GTX105914) dilution: 1:1000

REFERENCE

Application Reference

Lee KY et al. Hum Mol Genet 2022; 31 (18) : 3144-3160 Mice lacking MBNL1 and MBNL2 exhibit sudden cardiac death and molecular signatures recapitulating myotonic dystrophy.
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169