GeneTex
United States (US)

Calsequestrin 2 antibody

GTX105914_40002_IHC_M_19011506_159.jpg
GTX105914_IHC_19011506_960.jpg
GTX105914_40002_WB_19011506_693.jpg

Cat No. GTX105914

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

HCT116

Predict Reactivity

Rat, Rabbit, Bovine, Dog, Chicken(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.68mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Calsequestrin-2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

FLJ26321 antibody, FLJ93514 antibody, PDIB2 antibody, CASQ2 antibody, calsequestrin-2 antibody, "calsequestrin, cardiac muscle isoform antibody", "calsequestrin 2, fast-twitch, cardiac muscle antibody", calsequestrin 2 (cardiac muscle) antibody

Cellular Localization

Sarcoplasmic reticulum lumen

Background

The protein encoded by this gene specifies the cardiac muscle family member of the calsequestrin family. Calsequestrin is localized to the sarcoplasmic reticulum in cardiac and slow skeletal muscle cells. The protein is a calcium binding protein that stores calcium for muscle function. Mutations in this gene cause stress-induced polymorphic ventricular tachycardia, also referred to as catecholaminergic polymorphic ventricular tachycardia 2 (CPVT2), a disease characterized by bidirectional ventricular tachycardia that may lead to cardiac arrest. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
GTX105914_40002_IHC_M_19011506_159.jpg

GTX105914 IHC-P Image

Calsequestrin-2 antibody detects Calsequestrin-2 protein at cytosol on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
Calsequestrin-2 antibody (GTX105914) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105914_IHC_19011506_960.jpg

GTX105914 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft , using Calsequestrin-2(GTX105914) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105914_40002_WB_19011506_693.jpg

GTX105914 WB Image

Calsequestrin 2 antibody detects Calsequestrin 2 protein by western blot analysis.
A. 30 μg HCT116 whole cell lysate/extract
10 % SDS-PAGE
Calsequestrin 2 antibody (GTX105914) dilution: 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf