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Calsequestrin antibody [VIIID12]

Anti-Calsequestrin antibody [VIIID12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22824
Anti-Calsequestrin antibody [VIIID12] used in Western Blot (WB). GTX22824
Anti-Calsequestrin antibody [VIIID12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22824
Anti-Calsequestrin antibody [VIIID12] used in IHC (Paraffin sections) (IHC-P). GTX22824
Anti-Calsequestrin antibody [VIIID12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22824
Anti-Calsequestrin antibody [VIIID12] used in IHC (Paraffin sections) (IHC-P). GTX22824

Cat. No. GTX22824

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 VIIID12

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P, IP, IHC

Reactivity

 Human, Mouse, Rat, Rabbit, Dog, Chicken, Pig, Rodent
Package
100 μl ($399)
See all Calsequestrin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1,000
ICC/IF 1:50-1:500
IHC-P 1:20
IP Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Product Note

This antibody recognizes calsequestrin in both type I (slow) and type II (fast) skeletal muscle tissues.

PROPERTIES

Form

Liquid

Buffer

Ascites diluted with PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Rabbit

Immunogen

Purified rabbit skeletal muscle sarcoplasmic reticulum.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

calsequestrin 1

Cellular Localization

Endoplasmic reticulum,Mitochondrion matrix

Database

Research Area

DATA IMAGES

Anti-Calsequestrin antibody [VIIID12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22824

GTX22824 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX22824 Calsequestrin antibody [VIIID12]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC

Anti-Calsequestrin antibody [VIIID12] used in Western Blot (WB). GTX22824

GTX22824 WB Image

WB analysis of canine skeletal muscle extract using GTX22824 Calsequestrin antibody [VIIID12].

Anti-Calsequestrin antibody [VIIID12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22824

GTX22824 ICC/IF Image

ICC/IF analysis of MCF-7 cells using GTX22824 Calsequestrin antibody [VIIID12]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC

Anti-Calsequestrin antibody [VIIID12] used in IHC (Paraffin sections) (IHC-P). GTX22824

GTX22824 IHC-P Image

IHC-P analysis of human skeletal muscle tissue using GTX22824 Calsequestrin antibody [VIIID12].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

Anti-Calsequestrin antibody [VIIID12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22824

GTX22824 ICC/IF Image

ICC/IF analysis of U251 cells using GTX22824 Calsequestrin antibody [VIIID12]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC

Anti-Calsequestrin antibody [VIIID12] used in IHC (Paraffin sections) (IHC-P). GTX22824

GTX22824 IHC-P Image

IHC-P analysis of human heart tissue using GTX22824 Calsequestrin antibody [VIIID12].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399