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Calsequestrin antibody

Anti-Calsequestrin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23516
Anti-Calsequestrin antibody used in IHC (Paraffin sections) (IHC-P). GTX23516
Anti-Calsequestrin antibody used in IHC (Paraffin sections) (IHC-P). GTX23516
Anti-Calsequestrin antibody used in IHC (Paraffin sections) (IHC-P). GTX23516
Anti-Calsequestrin antibody used in Western Blot (WB). GTX23516

Cat No. GTX23516

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP, IHC
Reactivity Human, Mouse, Rat, Rabbit, Sheep, Dog
Package
100 μl ($319)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10,000
ICC/IF Assay dependent
IHC-P 1:100-1:500
IP Assay dependent
IHC Assay dependent
Not tested in other applications.

Specificity/Sensitivity

This antibody recognizes both cardiac and skeletal muscle calsequestrin.
PROPERTIES

Form

Liquid

Buffer

Serum diluted in PBS, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Dog

Immunogen

Purified canine cardiac calsequestrin.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
DATA IMAGES
Anti-Calsequestrin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23516

GTX23516 ICC/IF Image

ICC/IF analysis of C2C12 cells using GTX23516 Calsequestrin antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 incubated overnight at 4 °C

Anti-Calsequestrin antibody used in IHC (Paraffin sections) (IHC-P). GTX23516

GTX23516 IHC-P Image

IHC-P analysis of human heart tissue using GTX23516 Calsequestrin antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0) microwaved for 8-15 min
Dilution : 1:200

Anti-Calsequestrin antibody used in IHC (Paraffin sections) (IHC-P). GTX23516

GTX23516 IHC-P Image

IHC-P analysis of human skeletal muscle using GTX23516 Calsequestrin antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0) microwaved for 8-15 min
Dilution : 1:200

Anti-Calsequestrin antibody used in IHC (Paraffin sections) (IHC-P). GTX23516

GTX23516 IHC-P Image

IHC-P analysis of mouse heart tissue using GTX23516 Calsequestrin antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0) microwaved for 8-15 min
Dilution : 1:200

Anti-Calsequestrin antibody used in Western Blot (WB). GTX23516

GTX23516 WB Image

WB analysis of 25 ug of RD (Lane 1), L6 (Lane 2) and mouse heart (Lane 3) cell lysates using GTX23516 Calsequestrin antibody.
Dilution : 1:5000

REFERENCE
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REVIEW
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Package List Price ($)
$ 319