GeneTex

  • Country / Location Selection

United States (US)

Cannabinoid receptor 1 antibody [C1C2], Internal

Overexpression
Anti-Cannabinoid receptor 1 antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100219
Anti-Cannabinoid receptor 1 antibody [C1C2], Internal used in Western Blot (WB). GTX100219
Anti-Cannabinoid receptor 1 antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100219
Anti-Cannabinoid receptor 1 antibody [C1C2], Internal used in Western Blot (WB). GTX100219
Anti-Cannabinoid receptor 1 antibody [C1C2], Internal used in Western Blot (WB). GTX100219
Anti-Cannabinoid receptor 1 antibody [C1C2], Internal used in Western Blot (WB). GTX100219

Cat. No. GTX100219

Reference 2
3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Cannabinoid receptor 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Positive Control

HEK-293T , human CB1-transfected HEK-293T cells , Mouse brain , Rat2 , U87-MG

Predict Reactivity

Bovine, Cat, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Intracellular domain of human Cannabinoid Receptor 1. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_1080948

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cannabinoid receptor 1 , CANN6 , CB-R , CB1 , CB1A , CB1K5 , CB1R , CNR

Cellular Localization

Cell membrane

Background

This gene encodes a protein that is one of two cannabinoid receptors. The cannabinoids, principally delta-9-tetrahydrocannabinol and synthetic analogs, are psychoactive ingredients of marijuana. The cannabinoid receptors are members of the family of guanine-nucleotide-binding protein (G-protein) coupled receptors which inhibit adenylate cyclase activity in a dose-dependent, stereoselective and pertussis toxin-sensitive manner. The two receptors have been found to be involved in the cannabinoid-induced CNS effects (including alterations in mood and cognition) experienced by users of marijuana. Two transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Cannabinoid receptor 1 antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100219

GTX100219 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using CB1 (GTX100219) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Cannabinoid receptor 1 antibody [C1C2], Internal used in Western Blot (WB). GTX100219

GTX100219 WB Image

Cannabinoid Receptor 1 antibody [C1C2], Internal detects CNR1 protein by western blot analysis.
A. 30 μg U87-MG whole cell lysate/extract
10% SDS-PAGE
Cannabinoid Receptor 1 antibody [C1C2], Internal (GTX100219) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cannabinoid receptor 1 antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100219

GTX100219 IHC-P Image

Cannabinoid Receptor 1 antibody [C1C2], Internal detects Cannabinoid Receptor 1 protein by immunohistochemical analysis.
Samples: Paraffin-Embedded mouse retina.
Green: Cannabinoid Receptor 1 protein stained by Cannabinoid Receptor 1 antibody [C1C2], Internal (GTX100219) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Cannabinoid receptor 1 antibody [C1C2], Internal used in Western Blot (WB). GTX100219

GTX100219 WB Image

Cannabinoid Receptor 1 antibody [C1C2], Internal detects CNR1 protein by western blot analysis.
A. 30 μg Rat2 whole cell lysate/extract
10% SDS-PAGE
Cannabinoid Receptor 1 antibody [C1C2], Internal (GTX100219) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cannabinoid receptor 1 antibody [C1C2], Internal used in Western Blot (WB). GTX100219

GTX100219 WB Image

Sample (50 μg of whole cell lysate)
A: mouse brain
7.5% SDS PAGE
GTX100219 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cannabinoid receptor 1 antibody [C1C2], Internal used in Western Blot (WB). GTX100219

GTX100219 WB Image

Overexpression

Cannabinoid Receptor 1 antibody [C1C2], Internal detects CNR1 protein by western blot analysis.
A. 10 μg HEK-293T whole cell lysate/extract
B. 10 μg whole cell lysate/extract of human CB1-transfected HEK-293T cells
10% SDS-PAGE
Cannabinoid Receptor 1 antibody [C1C2], Internal (GTX100219) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Chang CH et al. Neurobiol Stress 2021; 15 : 100337 Regulation of stress-provoked aggressive behavior using endocannabinoids.
Application : WB
Reactivity : Mouse
Fugedi G et al. BMC Pregnancy Childbirth 2014; 14 (1) : 395 Increased placental expression of cannabinoid receptor 1 in preeclampsia: an observational study.
Application : IHC-P
Reactivity : Human
Submit a Reference

REVIEW

Cannabinoid receptor 1 antibody [C1C2], Internal Cat. No. GTX100219

Rating
( Average 4 based on 3 users reviews)
Application
Western Blot(WB)
( Average 4.5 based on 2 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 3 based on 1 users reviews)
Anti-Cannabinoid receptor 1 antibody [C1C2], Internal used in Western Blot (WB). GTX100219
Date : Anonymous submitted on 14-Feb-2023
Score :
Application Tested : WB
Sample Species : Hu
Sample : H460, H1299, and H1688 cell lysates
Blocking : HyBlock 1min Blocking Buffer, 25°C, 0.01Hr
Primary Antibody : 1:1000, 15°C, 4Hr
Anti-Cannabinoid receptor 1 antibody [C1C2], Internal used in Western Blot (WB). GTX100219
Date : Anonymous submitted on 01-Mar-2022
Score :
Application Tested : WB
Sample Species : Ms
Sample : mouse adipose tissue lysates
Amount used : 20μg
Blocking : 6% skim milk in TBS-T, 4°C, 1Hr
Primary Antibody : 1:5000, 4°C, 1Hr
Lane Description :
Lane 1: mouse subcutaneous adipose tissue lysate
Lane 2: mouse visceral adipose tissue lysate
Lane 3: mouse brown adipose tissue lysate
Lane 4: mouse liver tissue lysate
Anti-Cannabinoid receptor 1 antibody [C1C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100219
Date : Anonymous submitted on 04-Oct-2013
Score :
Application Tested : ICC/IF
Sample Species : Ms
Sample : NSC34 cells
Blocking : 3% NGS, 25°C, 2Hr
Fixation : PFA
Permeabilization /Antigen retrieval : NP40
Primary Antibody : 1:500, 4°C, 16Hr
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 399
$ 169