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Cannabinoid Receptor 1 antibody [C1C2], Internal

Overexpression
GTX100219_IHC_19011506_403.jpg
GTX100219_39422_WB_B_19011506_337.jpg
GTX100219_39422_WB_R_19011506_626.jpg
GTX100219_39422_20171017_IHC-P_M_19011506_200.jpg
GTX100219_39422_WB_19011506_507.jpg
GTX100219_39422_WB_M_Brain_19011506_422.jpg

Cat No. GTX100219

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Positive Control

U87-MG , human CB1-transfected HEK-293T cells (100ºC) , mouse brain , Rat2 , *NSC34 ,

Predict Reactivity

Bovine, Cat, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Cannabinoid Receptor 1. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CANN6 antibody, CB-R antibody, CB1 antibody, CB1A antibody, CB1K5 antibody, CB1R antibody, CNR antibody, CNR1 antibody, central cannabinoid receptor antibody, cannabinoid receptor 1 antibody, cannabinoid receptor 1 (brain) antibody

Cellular Localization

Cell membrane

Background

This gene encodes a protein that is one of two cannabinoid receptors. The cannabinoids, principally delta-9-tetrahydrocannabinol and synthetic analogs, are psychoactive ingredients of marijuana. The cannabinoid receptors are members of the family of guanine-nucleotide-binding protein (G-protein) coupled receptors which inhibit adenylate cyclase activity in a dose-dependent, stereoselective and pertussis toxin-sensitive manner. The two receptors have been found to be involved in the cannabinoid-induced CNS effects (including alterations in mood and cognition) experienced by users of marijuana. Two transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100219_IHC_19011506_403.jpg

GTX100219 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using CB1 (GTX100219) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100219_39422_WB_B_19011506_337.jpg

GTX100219 WB Image

Overexpression

Cannabinoid Receptor 1 antibody [C1C2], Internal detects CNR1 protein by western blot analysis.
A. 10 µg HEK-293T whole cell lysate/extract
B. 10 µg whole cell lysate/extract of human CB1-transfected HEK-293T cells
10% SDS-PAGE
Cannabinoid Receptor 1 antibody [C1C2], Internal (GTX100219) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100219_39422_WB_R_19011506_626.jpg

GTX100219 WB Image

Cannabinoid Receptor 1 antibody [C1C2], Internal detects CNR1 protein by western blot analysis.
A. 30 µg Rat2 whole cell lysate/extract
10% SDS-PAGE
Cannabinoid Receptor 1 antibody [C1C2], Internal (GTX100219) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100219_39422_20171017_IHC-P_M_19011506_200.jpg

GTX100219 IHC-P Image

Cannabinoid Receptor 1 antibody [C1C2], Internal detects Cannabinoid Receptor 1 protein by immunohistochemical analysis.
Samples: Paraffin-Embedded mouse retina.
Green: Cannabinoid Receptor 1 protein stained by Cannabinoid Receptor 1 antibody [C1C2], Internal (GTX100219) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX100219_39422_WB_19011506_507.jpg

GTX100219 WB Image

Cannabinoid Receptor 1 antibody [C1C2], Internal detects CNR1 protein by western blot analysis.
A. 30 µg U87-MG whole cell lysate/extract
10% SDS-PAGE
Cannabinoid Receptor 1 antibody [C1C2], Internal (GTX100219) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100219_39422_WB_M_Brain_19011506_422.jpg

GTX100219 WB Image

Sample (50 µg of whole cell lysate)
A: mouse brain
7.5% SDS PAGE
GTX100219 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

Cannabinoid Receptor 1 antibody [C1C2], Internal Cat No. GTX100219

Rating ( Average 3 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 3 based on 1 users reviews)
Date : Anonymous submitted on 04-Oct-2013
Score :
Application Tested : ICC/IF
Sample Species : Ms
Sample : NSC34 cells
Blocking : 3% NGS, 25°C, 2Hr
Fixation : PFA
Permeabilization /Antigen retrieval : NP40
Primary Antibody : 1:500, 4°C, overnightHr
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 319
$ 169