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Cannabinoid receptor 1 antibody [C1C2], Internal

Anti-Cannabinoid receptor 1 antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100219
Anti-Cannabinoid receptor 1 antibody [C1C2], Internal used in Western Blot (WB). GTX100219
Anti-Cannabinoid receptor 1 antibody [C1C2], Internal used in Western Blot (WB). GTX100219
Anti-Cannabinoid receptor 1 antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100219
Anti-Cannabinoid receptor 1 antibody [C1C2], Internal used in Western Blot (WB). GTX100219
Anti-Cannabinoid receptor 1 antibody [C1C2], Internal used in Western Blot (WB). GTX100219

Cat No. GTX100219

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Positive Control

U87-MG , human CB1-transfected HEK-293T cells (100ºC) , mouse brain , Rat2 , *NSC34 ,

Predict Reactivity

Bovine, Cat, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Cannabinoid Receptor 1. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_1080948

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cannabinoid receptor 1 , CANN6 , CB-R , CB1 , CB1A , CB1K5 , CB1R , CNR

Cellular Localization

Cell membrane

Background

This gene encodes a protein that is one of two cannabinoid receptors. The cannabinoids, principally delta-9-tetrahydrocannabinol and synthetic analogs, are psychoactive ingredients of marijuana. The cannabinoid receptors are members of the family of guanine-nucleotide-binding protein (G-protein) coupled receptors which inhibit adenylate cyclase activity in a dose-dependent, stereoselective and pertussis toxin-sensitive manner. The two receptors have been found to be involved in the cannabinoid-induced CNS effects (including alterations in mood and cognition) experienced by users of marijuana. Two transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Cannabinoid receptor 1 antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100219

GTX100219 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using CB1 (GTX100219) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Cannabinoid receptor 1 antibody [C1C2], Internal used in Western Blot (WB). GTX100219

GTX100219 WB Image

Cannabinoid Receptor 1 antibody [C1C2], Internal detects CNR1 protein by western blot analysis.
A. 10 μg HEK-293T whole cell lysate/extract
B. 10 μg whole cell lysate/extract of human CB1-transfected HEK-293T cells
10% SDS-PAGE
Cannabinoid Receptor 1 antibody [C1C2], Internal (GTX100219) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cannabinoid receptor 1 antibody [C1C2], Internal used in Western Blot (WB). GTX100219

GTX100219 WB Image

Cannabinoid Receptor 1 antibody [C1C2], Internal detects CNR1 protein by western blot analysis.
A. 30 μg Rat2 whole cell lysate/extract
10% SDS-PAGE
Cannabinoid Receptor 1 antibody [C1C2], Internal (GTX100219) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cannabinoid receptor 1 antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100219

GTX100219 IHC-P Image

Cannabinoid Receptor 1 antibody [C1C2], Internal detects Cannabinoid Receptor 1 protein by immunohistochemical analysis.
Samples: Paraffin-Embedded mouse retina.
Green: Cannabinoid Receptor 1 protein stained by Cannabinoid Receptor 1 antibody [C1C2], Internal (GTX100219) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Cannabinoid receptor 1 antibody [C1C2], Internal used in Western Blot (WB). GTX100219

GTX100219 WB Image

Cannabinoid Receptor 1 antibody [C1C2], Internal detects CNR1 protein by western blot analysis.
A. 30 μg U87-MG whole cell lysate/extract
10% SDS-PAGE
Cannabinoid Receptor 1 antibody [C1C2], Internal (GTX100219) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cannabinoid receptor 1 antibody [C1C2], Internal used in Western Blot (WB). GTX100219

GTX100219 WB Image

Sample (50 μg of whole cell lysate)
A: mouse brain
7.5% SDS PAGE
GTX100219 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

Cannabinoid receptor 1 antibody [C1C2], Internal Cat No. GTX100219

Rating ( Average 3.5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 3 based on 1 users reviews)
Date : Anonymous submitted on 01-Mar-2022
Score :
Application Tested : WB
Sample Species : Ms
Sample : mouse adipose tissue lysates
Amount used : 20μg
Blocking : 6% skim milk in TBS-T, 4°C, 1Hr
Primary Antibody : 1:5000, 4°C, 1Hr
Lane Description :
Lane 1: mouse subcutaneous adipose tissue lysate
Lane 2: mouse visceral adipose tissue lysate
Lane 3: mouse brown adipose tissue lysate
Lane 4: mouse liver tissue lysate
Date : Anonymous submitted on 04-Oct-2013
Score :
Application Tested : ICC/IF
Sample Species : Ms
Sample : NSC34 cells
Blocking : 3% NGS, 25°C, 2Hr
Fixation : PFA
Permeabilization /Antigen retrieval : NP40
Primary Antibody : 1:500, 4°C, 16Hr
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 379
$ 169