GeneTex
United States (US)

Carbonic Anhydrase 1 antibody, Internal

GTX88385_994_IHC-P_18122102_544.jpg
GTX88385_2393_WB_18122102_738.jpg

Cat No. GTX88385

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.03-0.1μg/ml
IHC-P 2.5μg/ml
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88385-PEP).

Predict Reactivity

Mouse, Rat(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-QAIKTKGKRAP, from the internal region of the protein sequence according to NP_001729.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Carbonic Anhydrase 1,Ca-I,Cab,Car1,Hel-S-11,Ca1

Background

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. This CA1 gene is closely linked to the CA2 and CA3 genes on chromosome 8. It encodes a cytosolic protein that is found at the highest level in erythrocytes. Allelic variants of this gene have been described in some populations. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

Database

Research Area

DATA IMAGES
GTX88385_994_IHC-P_18122102_544.jpg

GTX88385 IHC-P Image

IHC-P analysis of human spleen using GTX88385 Carbonic Anhydrase 1 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 2.5µg/ml

GTX88385_2393_WB_18122102_738.jpg

GTX88385 WB Image

WB analysis of human liver lysate using GTX88385 Carbonic Anhydrase 1 antibody, Internal.
Dilution : 0.03µg/ml
Loading : 35µg protein in RIPA buffer

Package List Price ($)
$ 329