APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
0.03-0.1μg/ml |
2.5μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX88385-PEP).
Predict Reactivity
Mouse, Rat(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-QAIKTKGKRAP, from the internal region of the protein sequence according to NP_001729.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
carbonic anhydrase 1 , CA-I , CAB , Car1 , HEL-S-11
Cellular Localization
Cytoplasm
Background
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. This CA1 gene is closely linked to the CA2 and CA3 genes on chromosome 8. It encodes a cytosolic protein that is found at the highest level in erythrocytes. Allelic variants of this gene have been described in some populations. Alternative splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Nov 2016]
Database
Research Area
DATA IMAGES
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GTX88385 WB Image
WB analysis of human liver lysate using GTX88385 Carbonic Anhydrase 1 antibody, Internal. Dilution : 0.03μg/ml Loading : 35μg protein in RIPA buffer
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GTX88385 IHC-P Image
IHC-P analysis of human spleen using GTX88385 Carbonic Anhydrase 1 antibody, Internal. Antigen retrieval : citrate buffer pH 6 Dilution : 2.5μg/ml
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REFERENCE
There are currently no references for Carbonic Anhydrase 1 antibody, Internal (GTX88385). Be the first to share your publications with this product.
REVIEW
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