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Carbonic Anhydrase 1 antibody

Anti-Carbonic Anhydrase 1 antibody used in Western Blot (WB). GTX132070

Cat No. GTX132070

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

mouse spleen

Predict Reactivity

Human, Dog, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.56 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Carbonic Anhydrase 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

carbonic anhydrase 1,CA-I,CAB,Car1,HEL-S-11

Cellular Localization

Cytoplasm

Background

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. This CA1 gene is closely linked to the CA2 and CA3 genes on chromosome 8. It encodes a cytosolic protein that is found at the highest level in erythrocytes. Allelic variants of this gene have been described in some populations. Alternative splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Nov 2016]

Database

Research Area

DATA IMAGES
Anti-Carbonic Anhydrase 1 antibody used in Western Blot (WB). GTX132070

GTX132070 WB Image

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Carbonic Anhydrase I antibody (GTX132070) diluted at 1:1000.

REFERENCE
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REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf