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Carbonic Anhydrase 1 antibody

Anti-Carbonic Anhydrase 1 antibody used in Western Blot (WB). GTX53905
Anti-Carbonic Anhydrase 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX53905

Cat. No. GTX53905

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all Carbonic Anhydrase 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:10 - 1:100
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-261 of human CA1 (NP_001122303.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

carbonic anhydrase 1 , CA-I , CAB , Car1 , HEL-S-11

Cellular Localization

Cytoplasm

Background

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. This CA1 gene is closely linked to the CA2 and CA3 genes on chromosome 8. It encodes a cytosolic protein that is found at the highest level in erythrocytes. Allelic variants of this gene have been described in some populations. Alternative splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Nov 2016]

Database

Research Area

DATA IMAGES

Anti-Carbonic Anhydrase 1 antibody used in Western Blot (WB). GTX53905

GTX53905 WB Image

WB analysis of various sample lysates using GTX53905 Carbonic Anhydrase 1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-Carbonic Anhydrase 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX53905

GTX53905 ICC/IF Image

ICC/IF analysis of A549 cells using GTX53905 Carbonic Anhydrase 1 antibody.
Blue : DAPI

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399