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Carbonic Anhydrase II antibody [N1C3]

Anti-Carbonic Anhydrase II antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX105562
Anti-Carbonic Anhydrase II antibody [N1C3] used in Western Blot (WB). GTX105562

Cat. No. GTX105562

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($419),
25 μl ($169)
See all Carbonic Anhydrase II products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

A431

Predict Reactivity

Rabbit, Bovine, Dog, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.17 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Carbonic anhydrase II. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036427

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

carbonic anhydrase 2 , CA-II , CAC , CAII , Car2 , HEL-76 , HEL-S-282

Cellular Localization

Cytoplasm

Background

CA2 is one of several (at least 7) isozymes of carbonic anhydrase. Carbonic anhydrase catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Carbonic Anhydrase II antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX105562

GTX105562 IHC-P Image

Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft , using Carbonic anhydrase 2(GTX105562) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Carbonic Anhydrase II antibody [N1C3] used in Western Blot (WB). GTX105562

GTX105562 WB Image

Sample (30 ug of whole cell lysate)
A: A431
12% SDS PAGE
GTX105562 diluted at 1:1000

REFERENCE

Application Reference

Wang B et al. BMC Cancer 2023; 23 (1) : 39 Identification and clinical validation of key genes as the potential biomarkers in colorectal adenoma.
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 419
$ 169