GeneTex
United States (US)

Carbonic Anhydrase II antibody [N1C3]

GTX105562_40359_WB_19011506_704.jpg
GTX105562_IHC_19011506_484.jpg

Cat No. GTX105562

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

A431

Predict Reactivity

Rabbit, Bovine, Dog, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.17 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Carbonic anhydrase II. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Carbonic Anhydrase 2,Ca-Ii,Cac,Caii,Car2,Hel-76,Hel-S-282,Ca2

Cellular Localization

Cytoplasm

Background

CA2 is one of several (at least 7) isozymes of carbonic anhydrase. Carbonic anhydrase catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX105562_40359_WB_19011506_704.jpg

GTX105562 WB Image

Sample (30 ug of whole cell lysate)
A: A431
12% SDS PAGE
GTX105562 diluted at 1:1000

GTX105562_IHC_19011506_484.jpg

GTX105562 IHC-P Image

Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft , using Carbonic anhydrase 2(GTX105562) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169