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Carbonic Anhydrase II antibody

Anti-Carbonic Anhydrase II antibody used in IHC (Paraffin sections) (IHC-P). GTX26621
Anti-Carbonic Anhydrase II antibody used in Western Blot (WB). GTX26621

Cat. No. GTX26621

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse
Package
1 mg ($399)
See all Carbonic Anhydrase II products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000-1:10000
IHC-P Assay dependent
ELISA 1:165000
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

11 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carbonic Anhydrase II collected from human erythrocytes

Purification

IgG fraction
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

carbonic anhydrase 2 , CA-II , CAC , CAII , Car2 , HEL-76 , HEL-S-282

Cellular Localization

Cytoplasm,Cell membrane

Background

CA2 is one of several (at least 7) isozymes of carbonic anhydrase. Carbonic anhydrase catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Carbonic Anhydrase II antibody used in IHC (Paraffin sections) (IHC-P). GTX26621

GTX26621 IHC-P Image

IHC-P analysis of various tissues using GTX26621 Carbonic Anhydrase II antibody.
Panel A : Negative control
Panel B : Human kidney tissue section
Panel C : Mouse eye tissue section
Antigen retrieval : Heat-mediated buffer (pH 6.2)
Dilution : 4 μg/mL

Anti-Carbonic Anhydrase II antibody used in Western Blot (WB). GTX26621

GTX26621 WB Image

WB analysis of human Carbonic Anhydrase II protein using GTX26621 Carbonic Anhydrase II antibody.
Loading : 50 ng
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399