GeneTex
United States (US)

Carbonic Anhydrase IX antibody [CA9/781]

Anti-Carbonic Anhydrase IX antibody [CA9/781] used in Western Blot (WB). GTX34452
Anti-Carbonic Anhydrase IX antibody [CA9/781] used in IHC (Paraffin sections) (IHC-P). GTX34452

Cat No. GTX34452

Host Mouse
Clonality Monoclonal
Clone Name CA9/781
Isotype IgG2b
Application WB, ICC/IF, IHC-P
Reactivity Human, Horse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-1ug/ml
ICC/IF 1-2ug/ml
IHC-P 0.5-1ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Specificity/Sensitivity

Recognizes a glycoprotein of ~200kDa, identified as carbonic anhydrase IX (CAIX/gp200). Carbonic Anhydrases (CAs) are members of a large family of zinc metallo-enzymes that catalyze the reversible hydration of carbon dioxide. CAs are involved in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption and the formation of aqueous humor, cerebrospinal fluid, saliva and gastric juice. They show extensive diversity in distribution and in their subcellular localization. CA IX is specifically expressed in clear-cell renal carcinomas.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant human CA9 protein

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Carbonic Anhydrase 9,Caix,Mn,Ca9

Background

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and is one of only two tumor-associated carbonic anhydrase isoenzymes known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12. [provided by RefSeq, Jun 2014]

Database

Research Area

DATA IMAGES
Anti-Carbonic Anhydrase IX antibody [CA9/781] used in Western Blot (WB). GTX34452

GTX34452 WB Image

Western Blot Analysis of HCT116 Cell Lysate using CAIX Monoclonal Antibody (CA9/781).

Anti-Carbonic Anhydrase IX antibody [CA9/781] used in IHC (Paraffin sections) (IHC-P). GTX34452

GTX34452 IHC-P Image

Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with CAIX Monoclonal Antibody (CA9/781).

Package List Price ($)
$ 329