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Carbonic Anhydrase IX antibody [CA9/781]

Anti-Carbonic Anhydrase IX antibody [CA9/781] used in Western Blot (WB). GTX34452
Anti-Carbonic Anhydrase IX antibody [CA9/781] used in IHC (Paraffin sections) (IHC-P). GTX34452

Cat. No. GTX34452

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 CA9/781

Isotype

 IgG2b

Application

 WB, IHC-P

Reactivity

 Human, Horse
Package
100 μg ($529)
See all Carbonic Anhydrase IX products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human CA9 protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CA9 , CAIX , MN , carbonic anhydrase 9 , Carbonic Anhydrase IX

Cellular Localization

Nucleus,Nucleolus,Cell membrane,Cell projection,Microvillus membrane

Background

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and is one of only two tumor-associated carbonic anhydrase isoenzymes known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12. [provided by RefSeq, Jun 2014]

Database

Research Area

DATA IMAGES

Anti-Carbonic Anhydrase IX antibody [CA9/781] used in Western Blot (WB). GTX34452

GTX34452 WB Image

WB analysis of HCT116 cell lysate using GTX34452 Carbonic Anhydrase IX antibody [CA9/781].

Anti-Carbonic Anhydrase IX antibody [CA9/781] used in IHC (Paraffin sections) (IHC-P). GTX34452

GTX34452 IHC-P Image

IHC-P analysis of human renal cell carcinoma tissue using GTX34452 Carbonic Anhydrase IX antibody [CA9/781].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529