Carbonic Anhydrase IX antibody [GT14212] detects Carbonic Anhydrase IX protein at membrane by immunofluorescent analysis.
Sample: 200μM CoCl2 for 48 hr A431 cells were fixed in ice-cold MeOH for 5 min.
Green: Carbonic Anhydrase IX protein stained by Carbonic Anhydrase IX antibody [GT14212] (GTX629747) diluted at 1:1000.
Blue: Hoechst 33342 staining.

Carbonic Anhydrase IX antibody [GT14212] detects Carbonic Anhydrase IX protein by Western blot analysis.
A. 30 μg HeLa whole cell lysate/extract (untreated)
B. 30 μg HeLa whole cell lysate/extract (200 μM CoCl2 treatment for 24 hr)
C. 30u g HeLa whole cell lysate/extract (200 μM CoCl2 treatment for 48 hr)
D. 30 μg HeLa whole cell lysate/extract (untreated)
E. 30 μg HeLa whole cell lysate/extract (1% O2 treatment for 24 hr)
F. 30 μg HeLa whole cell lysate/extract (1% O2 treatment for 48 hr)
7.5 % SDS-PAGE
Carbonic Anhydrase IX antibody [GT14212] (GTX629747) dilution: 1:1000