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Carbonic Anhydrase IX antibody [HL1410]

Anti-Carbonic Anhydrase IX antibody [HL1410] used in IHC (Paraffin sections) (IHC-P). GTX636876
Anti-Carbonic Anhydrase IX antibody [HL1410] used in IHC (Paraffin sections) (IHC-P). GTX636876
Anti-Carbonic Anhydrase IX antibody [HL1410] used in Western Blot (WB). GTX636876
Anti-Carbonic Anhydrase IX antibody [HL1410] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636876

Cat. No. GTX636876

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1410

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Observed MW

50-58 kDa.

Product Note

This antibody was raised against human Carbonic Anhydrase IX Extracellular domain.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human Carbonic Anhydrase IX. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

carbonic anhydrase 9 , CAIX , MN

Cellular Localization

Nucleus,Cell membrane,Nucleolus

Background

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and is one of only two tumor-associated carbonic anhydrase isoenzymes known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12. [provided by RefSeq, Jun 2014]

Database

Research Area

DATA IMAGES

Anti-Carbonic Anhydrase IX antibody [HL1410] used in IHC (Paraffin sections) (IHC-P). GTX636876

GTX636876 IHC-P Image

Carbonic Anhydrase IX antibody [HL1410] detects Carbonic Anhydrase IX protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
Carbonic Anhydrase IX stained by Carbonic Anhydrase IX antibody [HL1410] (GTX636876) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Carbonic Anhydrase IX antibody [HL1410] used in IHC (Paraffin sections) (IHC-P). GTX636876

GTX636876 IHC-P Image

Carbonic Anhydrase IX antibody [HL1410] detects Carbonic Anhydrase IX protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
Carbonic Anhydrase IX stained by Carbonic Anhydrase IX antibody [HL1410] (GTX636876) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Carbonic Anhydrase IX antibody [HL1410] used in Western Blot (WB). GTX636876

GTX636876 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Carbonic Anhydrase IX antibody [HL1410] (GTX636876) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Carbonic Anhydrase IX antibody [HL1410] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636876

GTX636876 ICC/IF Image

Carbonic Anhydrase IX antibody [HL1410] detects Carbonic Anhydrase IX protein at cell membrane by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Carbonic Anhydrase IX stained by Carbonic Anhydrase IX antibody [HL1410] (GTX636876) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10μm.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations