APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000-1:10000 |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Observed MW
50-58 kDa.
Product Note
This antibody was raised against human Carbonic Anhydrase IX Extracellular domain.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the Extracellular domain of human Carbonic Anhydrase IX. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
carbonic anhydrase 9 , CAIX , MN
Cellular Localization
Nucleus,Cell membrane,Nucleolus
Background
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and is one of only two tumor-associated carbonic anhydrase isoenzymes known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12. [provided by RefSeq, Jun 2014]
Database
Research Area
DATA IMAGES
![Anti-Carbonic Anhydrase IX antibody [HL1410] used in IHC (Paraffin sections) (IHC-P). GTX636876](/templates/default/js/lightbox/images/loading.gif "Anti-Carbonic Anhydrase IX antibody [HL1410] used in IHC (Paraffin sections) (IHC-P). GTX636876")
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GTX636876 IHC-P Image
Carbonic Anhydrase IX antibody [HL1410] detects Carbonic Anhydrase IX protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissues.Carbonic Anhydrase IX stained by Carbonic Anhydrase IX antibody [HL1410] (GTX636876) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636876 IHC-P Image
Carbonic Anhydrase IX antibody [HL1410] detects Carbonic Anhydrase IX protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.Carbonic Anhydrase IX stained by Carbonic Anhydrase IX antibody [HL1410] (GTX636876) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636876 WB Image
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Carbonic Anhydrase IX antibody [HL1410] (GTX636876) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636876 ICC/IF Image
Carbonic Anhydrase IX antibody [HL1410] detects Carbonic Anhydrase IX protein at cell membrane by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: Carbonic Anhydrase IX stained by Carbonic Anhydrase IX antibody [HL1410] (GTX636876) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar= 10μm.
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REFERENCE
There are currently no references for Carbonic Anhydrase IX antibody [HL1410] (GTX636876). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Carbonic Anhydrase IX antibody [HL1410] (GTX636876). Be the first to share your experience with this product.
![Anti-Carbonic Anhydrase IX antibody [HL1410] used in IHC (Paraffin sections) (IHC-P). GTX636876](/upload/website/prouct_img/small/GTX636876/GTX636876_44732_20221230_IHC-P_multiple_R_22122821_863.webp "Anti-Carbonic Anhydrase IX antibody [HL1410] used in IHC (Paraffin sections) (IHC-P). GTX636876")
![Anti-Carbonic Anhydrase IX antibody [HL1410] used in IHC (Paraffin sections) (IHC-P). GTX636876](/upload/website/prouct_img/small/GTX636876/GTX636876_44732_20221230_IHC-P_multiple_M_22122821_799.webp "Anti-Carbonic Anhydrase IX antibody [HL1410] used in IHC (Paraffin sections) (IHC-P). GTX636876")
![Anti-Carbonic Anhydrase IX antibody [HL1410] used in Western Blot (WB). GTX636876](/upload/website/prouct_img/small/GTX636876/GTX636876_T-44620_20220520_WB_treatment_CoCl2_w_23061202_434.webp "Anti-Carbonic Anhydrase IX antibody [HL1410] used in Western Blot (WB). GTX636876")
![Anti-Carbonic Anhydrase IX antibody [HL1410] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636876](/upload/website/prouct_img/small/GTX636876/GTX636876_T-44620_20220826_ICC_IF_22083119_865.webp "Anti-Carbonic Anhydrase IX antibody [HL1410] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636876")
![Anti-Carbonic Anhydrase IX antibody [HL1410] used in IHC (Paraffin sections) (IHC-P). GTX636876](/upload/website/prouct_img/normal/GTX636876/GTX636876_44732_20221230_IHC-P_multiple_R_22122821_863.webp "Anti-Carbonic Anhydrase IX antibody [HL1410] used in IHC (Paraffin sections) (IHC-P). GTX636876")
![Anti-Carbonic Anhydrase IX antibody [HL1410] used in IHC (Paraffin sections) (IHC-P). GTX636876](/upload/website/prouct_img/normal/GTX636876/GTX636876_44732_20221230_IHC-P_multiple_M_22122821_799.webp "Anti-Carbonic Anhydrase IX antibody [HL1410] used in IHC (Paraffin sections) (IHC-P). GTX636876")
![Anti-Carbonic Anhydrase IX antibody [HL1410] used in Western Blot (WB). GTX636876](/upload/website/prouct_img/normal/GTX636876/GTX636876_T-44620_20220520_WB_treatment_CoCl2_w_23061202_434.webp "Anti-Carbonic Anhydrase IX antibody [HL1410] used in Western Blot (WB). GTX636876")
![Anti-Carbonic Anhydrase IX antibody [HL1410] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636876](/upload/website/prouct_img/normal/GTX636876/GTX636876_T-44620_20220826_ICC_IF_22083119_865.webp "Anti-Carbonic Anhydrase IX antibody [HL1410] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636876")