GeneTex
United States (US)

Carbonic Anhydrase IX antibody

Orthogonal
GTX128428_41500_20160307_IHC-P_18121410_544.jpg
GTX128428_41500_IHC_18121410_846.jpg
GTX128428_41500_WB_CoCl2_18121410_186.jpg
GTX128428_41500_IFA_18121410_194.jpg

Cat No. GTX128428

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

HepG2 (untreated) , HepG2 ( 200uM CoCl2 treatment for 24hr)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Carbonic Anhydrase IX. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CAIX Antibody, carbonic anhydrase IX Antibody, MN Antibody, CA9 Antibody

Cellular Localization

Nucleus , nucleolus , Cell membrane , Cell projection , microvillus membrane

Background

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and the only tumor-associated carbonic anhydrase isoenzyme known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX128428_41500_20160307_IHC-P_18121410_544.jpg

GTX128428 IHC-P Image

Carbonic Anhydrase IX antibody detects Carbonic Anhydrase IX protein at cell membrane in human renal cell carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human renal cell carcinoma.
Carbonic Anhydrase IX antibody (GTX128428) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX128428_41500_IHC_18121410_846.jpg

GTX128428 IHC-P Image

Carbonic Anhydrase IX antibody detects Carbonic Anhydrase IX protein at membrane on human cervical carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded cervical carcinoma.
Carbonic Anhydrase IX antibody (GTX128428) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX128428_41500_WB_CoCl2_18121410_186.jpg

GTX128428 WB Image

Orthogonal

Carbonic Anhydrase IX antibody detects Carbonic Anhydrase IX protein by western blot analysis.
A. 30 µg HepG2 whole cell lysate/extract (untreated)
B. 30 µg HepG2 whole cell lysate/extract ( 200 µM CoCl2 treatment for 24 hr)
10% SDS-PAGE
Carbonic Anhydrase IX antibody (GTX128428) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX128428_41500_IFA_18121410_194.jpg

GTX128428 ICC/IF Image

Carbonic Anhydrase IX antibody detects Carbonic Anhydrase IX protein at membrane by immunofluorescent analysis.
Sample: 200 μM CoCl2 treated (right) or untreated (left) A431 cells were fixed in ice-cold MeOH for 5 min.
Green: Carbonic Anhydrase IX protein stained by Carbonic Anhydrase IX antibody (GTX128428) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169