GeneTex
United States (US)

Carbonic Anhydrase IX antibody

Overexpression
GTX15086_1084_WB_18121410_174.jpg
GTX15086_454_IHC-P_18121410_797.jpg
GTX15086_292_IHC-P_18121410_590.jpg
GTX15086_293_IHC_18121410_992.jpg
GTX15086_1086_WB_18121410_475.jpg
GTX15086_131_ICC-IF_18121410_253.jpg
GTX15086_1085_WB_18121410_745.jpg
GTX15086_1083_WB_18121410_907.jpg

Cat No. GTX15086

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr, FACS, ELISA, ChIP assay, GSA
Reactivity Human, Mouse, Rat, Dog
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:2000
ICC/IF 1:1000
IHC-P 1:1000
IHC-Fr 1:1000
FACS Assay dependent
ELISA Assay dependent
ChIP assay 1:10 - 1:500
GSA Assay dependent
Not tested in other applications.

Calculated MW

50 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS, 0.025% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide made to a C-terminal sequence of human Carbonic Anhydrase IX (within residues 400-459) [UniProt Q16790]

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Carbonic Anhydrase 9,Caix,Mn,Ca9

Cellular Localization

Nucleus,nucleolus,Cell membrane,Cell projection,microvillus membrane

Background

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and the only tumor-associated carbonic anhydrase isoenzyme known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX15086_1084_WB_18121410_174.jpg

GTX15086 WB Image

WB analysis of (1)HeLa, (2)MDA-MB-231 and (3)A549 cell lysate using GTX15086 Carbonic Anhydrase IX antibody.

GTX15086_454_IHC-P_18121410_797.jpg

GTX15086 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX15086 Carbonic Anhydrase IX antibody.
Dilution : 1:1000

GTX15086_292_IHC-P_18121410_590.jpg

GTX15086 IHC-P Image

IHC-P analysis of renal carcinoma tissue using GTX15086 Carbonic Anhydrase IX antibody.

GTX15086_293_IHC_18121410_992.jpg

GTX15086 IHC-Fr Image

IHC-Fr analysis of renal carcinoma tissue using GTX15086 Carbonic Anhydrase IX antibody.
Panel A : primary antibody
Panel B : staining with normal rabbit serum

GTX15086_1086_WB_18121410_475.jpg

GTX15086 WB Image

WB analysis of rat renal cortex tissue lysate using GTX15086 Carbonic Anhydrase IX antibody.

GTX15086_131_ICC-IF_18121410_253.jpg

GTX15086 ICC/IF Image

Overexpression

ICC/IF analysis of HEK 293 cells overexpressing Carbonic Anhydrase IX (Panel A) and mock cells (Panel B) using GTX15086 Carbonic Anhydrase IX antibody.

GTX15086_1085_WB_18121410_745.jpg

GTX15086 WB Image

WB analysis of HeLa cell lysate using GTX15086 Carbonic Anhydrase IX antibody.

GTX15086_1083_WB_18121410_907.jpg

GTX15086 WB Image

Overexpression

WB analysis of HEK overexpressing Carbonic Anhydrase IX cell lysate using GTX15086 Carbonic Anhydrase IX antibody. Rabbit IgG was used as a negative control.

REFERENCE
Package List Price ($)
$ 329