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Carbonic anhydrase protein, His tag (active)

Carbonic anhydrase protein, His tag (active). GTX66896-pro

Cat. No. GTX66896-pro

Application

 Functional Assay

Species

 E. coli
Package
20 μg ($219)
See all Carbonic anhydrase protein products

APPLICATION

Application Note

Specific activity is > 1000 pmol/min/μg, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37ºC.

PROPERTIES

Form

Liquid

Buffer

20mM Tris-HCl, 10% Glycerol, 1mM DTT

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Region/Sequence

Full length protein, N-terminal His-Tag; Length: 240 a.a. Sequence: MGSSHHHHHH SSGLVPRGSH MKDIDTLISN NALWSKMLVE EDPGFFEKLA QAQKPRFLWI GCSDSRVPAE RLTGLEPGEL FVHRNVANLV IHTDLNCLSV VQYAVDVLEV EHIIICGHYG CGGVQAAVEN PELGLINNWL LHIRDIWFKH SSLLGEMPQE RRLDTLCELN VMEQVYNLGH STIMQSAWKR GQKVTIHGWA YGIHDGLLRD LDVTATNRET LEQRYRHGIS NLKLKHANHK

Expression System

E. coli

Purity

> 95% by SDS-PAGE.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

carbonic anhydrase , ECK0125 , JW0122 , yadF

Database

Research Area

DATA IMAGES

Carbonic anhydrase protein, His tag (active). GTX66896-pro

GTX66896-pro Image

3 μg of GTX66896-pro Carbonic anhydrase protein (active) by SDS-PAGE under reducing condition and visualized by coomassie blue stain

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SDS
Glycerol.pdf
Package List Price ($)
$ 219