Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Carboxypeptidase B antibody (GTX105170) diluted at 1:500.

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Carboxypeptidase B antibody [N1C1] (GTX105170) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Carboxypeptidase B antibody [N1C1] (GTX105170) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.