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Carboxypeptidase B antibody [N3C3]

Overexpression
Anti-Carboxypeptidase B antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX114334
Anti-Carboxypeptidase B antibody [N3C3] used in Western Blot (WB). GTX114334
Anti-Carboxypeptidase B antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX114334
Anti-Carboxypeptidase B antibody [N3C3] used in Western Blot (WB). GTX114334
Anti-Carboxypeptidase B antibody [N3C3] used in Western Blot (WB). GTX114334
Anti-Carboxypeptidase B antibody [N3C3] used in Western Blot (WB). GTX114334

Cat. No. GTX114334

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Carboxypeptidase B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

rat pancreas , AsPC-1 , mouse pancreas , CPB1-transfected 293T

Predict Reactivity

Bovine, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.74 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Carboxypeptidase B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619759

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

carboxypeptidase B1 , CPB , PASP , PCPB

Cellular Localization

Secreted

Background

Three different procarboxypeptidases A and two different procarboxypeptidases B have been isolated. The B1 and B2 forms differ from each other mainly in isoelectric point. Carboxypeptidase B1 is a highly tissue-specific protein and is a useful serum marker for acute pancreatitis and dysfunction of pancreatic transplants. It is not elevated in pancreatic carcinoma. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Carboxypeptidase B antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX114334

GTX114334 IHC-P Image

Carboxypeptidase B antibody [N3C3] detects Carboxypeptidase B protein at cytoplasm in mouse pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
Carboxypeptidase B antibody [N3C3] (GTX114334) diluted at 1:1000.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Carboxypeptidase B antibody [N3C3] used in Western Blot (WB). GTX114334

GTX114334 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Carboxypeptidase B antibody [N3C3] (GTX114334) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Carboxypeptidase B antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX114334

GTX114334 IHC-P Image

Carboxypeptidase B antibody [N3C3] detects Carboxypeptidase B protein at cytoplasm in rat pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded rat pancreas.
Carboxypeptidase B antibody [N3C3] (GTX114334) diluted at 1:1000.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Carboxypeptidase B antibody [N3C3] used in Western Blot (WB). GTX114334

GTX114334 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Carboxypeptidase B antibody [N3C3] (GTX114334) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Carboxypeptidase B antibody [N3C3] used in Western Blot (WB). GTX114334

GTX114334 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Carboxypeptidase B antibody [N3C3] (GTX114334) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Carboxypeptidase B antibody [N3C3] used in Western Blot (WB). GTX114334

GTX114334 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Carboxypeptidase B antibody [N3C3] (GTX114334) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169