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Carboxypeptidase E antibody [N2C2], Internal

Anti-Carboxypeptidase E antibody [N2C2], Internal used in Western Blot (WB). GTX106177
Anti-Carboxypeptidase E antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX106177
Anti-Carboxypeptidase E antibody [N2C2], Internal used in Western Blot (WB). GTX106177

Cat. No. GTX106177

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Carboxypeptidase E products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Positive Control

Mouse brain , CPE transfected 293T

Predict Reactivity

Rat, Zebrafish, Bovine, Pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.68 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Carboxypeptidase E. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950027

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

carboxypeptidase E , CPH

Cellular Localization

Secreted

Background

This gene encodes a carboxypeptidase that cleaves C-terminal amino acid residues and is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin. It is a peripheral membrane protein. The protein specifically binds regulated secretory pathway proteins, including prohormones, but not constitutively secreted proteins. Mutations in this gene are implicated in type II diabetes. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Carboxypeptidase E antibody [N2C2], Internal used in Western Blot (WB). GTX106177

GTX106177 WB Image

Sample (20 μg of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX106177 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Carboxypeptidase E antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX106177

GTX106177 IHC-P Image

Carboxypeptidase E antibody [N2C2], Internal detects Carboxypeptidase E protein at nucleus on human breast cancer by immunohistochemical analysis.
Sample: Paraffin-embedded breast cancer.
Carboxypeptidase E antibody [N2C2], Internal (GTX106177) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Carboxypeptidase E antibody [N2C2], Internal used in Western Blot (WB). GTX106177

GTX106177 WB Image

Western Blot analysis of CPE expression in transfected 293T cell line by CPE polyclonal antibody.
A: Non-transfected lysate.
B: CPE transfected lysate.
12% SDS PAGE
GTX106177 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169