GeneTex
United States (US)

Carboxypeptidase M antibody [N1C2]

GTX106203_WB_19011506_277.jpg
GTX106203_39932_20151015_WB_R_liver_19011506_676.jpg

Cat No. GTX106203

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

HepG2 , Rat liver

Predict Reactivity

Mouse(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Carboxypeptidase M. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CPM antibody, carboxypeptidase M antibody

Cellular Localization

Cell membrane , GPI-anchor

Background

The protein encoded by this gene is a membrane-bound arginine/lysine carboxypeptidase. Its expression is associated with monocyte to macrophage differentiation. This encoded protein contains hydrophobic regions at the amino and carboxy termini and has 6 potential asparagine-linked glycosylation sites. The active site residues of carboxypeptidases A and B are conserved in this protein. Three alternatively spliced transcript variants encoding the same protein have been described for this gene. [provided by RefSeq]

Database

DATA IMAGES
GTX106203_WB_19011506_277.jpg

GTX106203 WB Image

Sample(30 ug whole cell lysate)
A:Hep G2 (GTX27900)
7.5% SDS PAGE
GTX106203 diluted at 1:500

GTX106203_39932_20151015_WB_R_liver_19011506_676.jpg

GTX106203 WB Image

Carboxypeptidase M antibody [N1C2] detects Carboxypeptidase M protein by western blot analysis. Rat tissue extracts (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Carboxypeptidase M antibody [N1C2] (GTX106203) diluted at 1:500.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169