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Carboxypeptidase Y antibody (HRP)

Anti-Carboxypeptidase Y antibody (HRP) used in Western Blot (WB). GTX40574
Anti-Carboxypeptidase Y antibody (HRP) used in Western Blot (WB). GTX40574
Anti-Carboxypeptidase Y antibody (HRP) used in Western Blot (WB). GTX40574

Cat. No. GTX40574

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ELISA, IHC

Reactivity

 Saccharomyces cerevisiae
Package
1 mg ($399)
See all Carboxypeptidase Y products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:5000
ELISA 1:14000
IHC Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl, 1% BSA

Preservative

0.01% Gentamicin Sulfate

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

10 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Saccharomyces cerevisiae

Immunogen

Carboxypeptidase Y (S. cerevisiae (Baker's Yeast)).

Purification

IgG fraction
This product is is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Conjugation

Horseradish peroxidase(HRP)

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

DATA IMAGES

Anti-Carboxypeptidase Y antibody (HRP) used in Western Blot (WB). GTX40574

GTX40574 WB Image

Sheep anti Lactoperoxidase antibody (GTX40571) was used to detect Lactoperoxidase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in blocking buffer). Detection shown was using Dylight 488 conjugated secondary antibody (1:10K in TBS/blocking buffer 1 hr RT).

Anti-Carboxypeptidase Y antibody (HRP) used in Western Blot (WB). GTX40574

GTX40574 WB Image

Western Blot of Peroxidase conjugated Rabbit anti-Carboxypeptidase Y Antibody (GTX40574). Lane 1: Carboxypeptidase Y. Lane 2: none. Load: 100 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase rabbit Carboxypeptidase Y antibody at 1:1,000 for 60 min at RT Block for 30 min at RT. Predicted/Observed size: 53 kDa for Carboxypeptidase Y. Other band(s): none.

Anti-Carboxypeptidase Y antibody (HRP) used in Western Blot (WB). GTX40574

GTX40574 WB Image

WB analysis of Saccharomyces cerevisiae caroxypeptidase protein using GTX40574 Carboxypeptidase Y antibody (HRP).
Loading : 100ng
Dilution : 1:1000

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SDS
PBS.pdf
Package List Price ($)
$ 399