GeneTex
United States (US)

Carboxypeptidase Y antibody (HRP)

Carboxypeptidase-Y-antibody-HRP_WB_GTX40574-1_18121409_254.jpg
Carboxypeptidase-Y-antibody-HRP_WB_GTX40574-2_18121409_330.jpg

Cat No. GTX40574

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, Dot, ELISA, IM
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
Dot Assay dependent
ELISA Assay dependent
IM Assay dependent
Not tested in other applications.

Specificity/Sensitivity

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Carboxypeptidase Y (Baker's Yeast).
PROPERTIES

Form

Liquid

Buffer

Preservative: 0.01% Gentamicin Sulfate. Constituents: PBS, 10mg/ml BSA. pH 7.2

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

10 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Carboxypeptidase Y (S. cerevisiae (Baker's Yeast)).

Purification


Purified from monospecific antiserum by delipidation, salt fractionation and ion exchange chromatography.

Conjugation

Horseradish peroxidase(HRP)

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Cellular Localization

Cytoplasmic
DATA IMAGES
Carboxypeptidase-Y-antibody-HRP_WB_GTX40574-1_18121409_254.jpg

GTX40574 WB Image

Western Blot of Peroxidase conjugated Rabbit anti-Carboxypeptidase Y Antibody (GTX40574). Lane 1: Carboxypeptidase Y. Lane 2: none. Load: 100 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase rabbit Carboxypeptidase Y antibody at 1:1,000 for 60 min at RT Block for 30 min at RT. Predicted/Observed size: 53 kDa for Carboxypeptidase Y. Other band(s): none.

Carboxypeptidase-Y-antibody-HRP_WB_GTX40574-2_18121409_330.jpg

GTX40574 WB Image

Sheep anti Lactoperoxidase antibody (GTX40571) was used to detect Lactoperoxidase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in blocking buffer). Detection shown was using Dylight 488 conjugated secondary antibody (1:10K in TBS/blocking buffer 1 hr RT).

Package List Price ($)
$ 289