WB analysis of Saccharomyces cerevisiae caroxypeptidase protein using GTX40574 Carboxypeptidase Y antibody (HRP).
Loading : 100ng
Dilution : 1:1000

Western Blot of Peroxidase conjugated Rabbit anti-Carboxypeptidase Y Antibody (GTX40574). Lane 1: Carboxypeptidase Y. Lane 2: none. Load: 100 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase rabbit Carboxypeptidase Y antibody at 1:1,000 for 60 min at RT Block for 30 min at RT. Predicted/Observed size: 53 kDa for Carboxypeptidase Y. Other band(s): none.

Sheep anti Lactoperoxidase antibody (GTX40571) was used to detect Lactoperoxidase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in blocking buffer). Detection shown was using Dylight 488 conjugated secondary antibody (1:10K in TBS/blocking buffer 1 hr RT).