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Carboxypeptidase Y antibody

Anti-Carboxypeptidase Y antibody used in Western Blot (WB). GTX48866

Cat. No. GTX48866

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ELISA

Reactivity

 Saccharomyces cerevisiae
Package
100 μl ($399)
See all Carboxypeptidase Y products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:3000
ELISA 1:5000 - 1:25000
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.02M Potassium Phosphate, 0.15M NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

90 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Saccharomyces cerevisiae

Immunogen

Carboxypeptidase Y [Baker's Yeast]

Purification

Antiserum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

carboxypeptidase C PRC1 , CPY1 , LBC1

Database

DATA IMAGES

Anti-Carboxypeptidase Y antibody used in Western Blot (WB). GTX48866

GTX48866 WB Image

Both the antiserum and IgG fractions of anti-Carboxypeptidase Y (Baker's Yeast) are shown to detect under reducing conditions of SDS-PAGE the 61,000 dalton enzyme in cellular extracts. Approximately 10 μg of total protein is loaded per lane. A 1:5,000 dilution of the primary antibody is used followed by detection using HRP Goat-anti-Rabbit IgG (H&L) diluted 1:4,000 and color development using 4-CN substrate until sufficient color develops. Other detection systems will yield similar results.

REFERENCE

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399