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United States (US)

Cardiac Troponin I antibody [16A11]

Cardiac-Troponin-I-antibody-16A11_Western blot_GTX10226-1_19011720_307.jpg
Cardiac-Troponin-I-antibody-16A11_ELISA_GTX10226-1_19011506_269.jpg

Cat No. GTX10226

Host Mouse
Clonality Monoclonal
Clone Name 16A11
Isotype IgG1
Application WB, ELISA, Apuri, IA
Reactivity Human, Rabbit, Goat, Bovine, Cat, Dog, Pig
See all Cardiac Troponin I products
APPLICATION

Application Note

This antibody is recommend for better sensitivity in WB. Recommended pair for ELISA: clone 19C7 (GTX28289) for capture and clone 16A11 (this antibody) for detection.

Calculated MW

24 kDa. ( Note )

Specificity/Sensitivity

reacts with free cardiac troponin I (cTnI) and cTnI forming complexes with other troponin components (in the presence of 5 mM EDTA). Not affected by heparin, phosphorylation, oxidation and troponin complex formation. Does not cross-react with skeletal muscle troponin I. Epitope:86 – 90 a.a of human cardiac Troponin I.
PROPERTIES

Form

Liquid

Buffer

PBS pH 7.4, 0.09 % sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

2mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Free human cardiac troponin I, native human cardiac troponin complex, or human cardiac troponin I peptides.

Purification

Protein A purified
Purity tested by electrophoresis.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Background

Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. It also binds to actin and tropomyosin.

Database

Research Area

DATA IMAGES
Cardiac-Troponin-I-antibody-16A11_Western blot_GTX10226-1_19011720_307.jpg

GTX10226 WB Image

Detection of cardiac troponin I (cTnI) by Western blotting. After SDS-PAGE cTnI was transferred to a nitrocellulose membrane and probed by Cardiac Troponin I antibody [16A11] (GTX10226) and then by a HRP-conjugated goat anti-mouse IgG antibody. Substrate - diaminobenzidine. Lane 1: 3 ng cTnI; Lane 2: 10 ng cTnI; Lane 3: 30 ng cTnI; Lane 4: 100 ng cTnI.
Cardiac-Troponin-I-antibody-16A11_ELISA_GTX10226-1_19011506_269.jpg

GTX10226 ELISA Image

Cardiac troponin I measurements using sandwich immunoassays to determine the total cardiac troponin I in serial blood samples from AMI patient.
Package List Price ($)
$ 289