APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| Assay dependent |
| Assay dependent |
| Assay dependent |
| Assay dependent |
| Assay dependent |
| Assay dependent |
| Assay dependent |
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Note :
Sandwich ELISA
Capture : GTX28289, Detection : GTX10226
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Not tested in other applications.
Calculated MW
Product Note
No cross-reactivity with skeletal muscle TnI.
PROPERTIES
Form
Liquid
Buffer
PBS pH7.4
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Free human cardiac troponin I, native human cardiac troponin complex, or human cardiac troponin I peptides.
Purification
Protein A purified
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
troponin I3, cardiac type , CMD1FF , CMD2A , CMH7 , RCM1 , TNNC1 , cTnI
Background
Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-Cardiac Troponin I antibody [16A11] used in ELISA (ELISA). GTX10226](/templates/default/js/lightbox/images/loading.gif "Anti-Cardiac Troponin I antibody [16A11] used in ELISA (ELISA). GTX10226")
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GTX10226 ELISA Image
Cardiac troponin I measurements using sandwich immunoassays to determine the total cardiac troponin I in serial blood samples from AMI patient.
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GTX10226 WB Image
Detection of cardiac troponin I (cTnI) by Western blotting. After SDS-PAGE cTnI was transferred to a nitrocellulose membrane and probed by Cardiac Troponin I antibody [16A11] (GTX10226) and then by a HRP-conjugated goat anti-mouse IgG antibody. Substrate - diaminobenzidine. Lane 1: 3 ng cTnI; Lane 2: 10 ng cTnI; Lane 3: 30 ng cTnI; Lane 4: 100 ng cTnI.
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REFERENCE
There are currently no references for Cardiac Troponin I antibody [16A11] (GTX10226). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Cardiac Troponin I antibody [16A11] (GTX10226). Be the first to share your experience with this product.
![Anti-Cardiac Troponin I antibody [16A11] used in ELISA (ELISA). GTX10226](/upload/website/prouct_img/small/GTX10226/Cardiac-Troponin-I-antibody-16A11_ELISA_GTX10226-1_19011506_269.jpg "Anti-Cardiac Troponin I antibody [16A11] used in ELISA (ELISA). GTX10226")
![Anti-Cardiac Troponin I antibody [16A11] used in Western Blot (WB). GTX10226](/upload/website/prouct_img/small/GTX10226/Cardiac-Troponin-I-antibody-16A11_Western blot_GTX10226-1_19011720_307.jpg "Anti-Cardiac Troponin I antibody [16A11] used in Western Blot (WB). GTX10226")
![Anti-Cardiac Troponin I antibody [16A11] used in ELISA (ELISA). GTX10226](/upload/website/prouct_img/normal/GTX10226/Cardiac-Troponin-I-antibody-16A11_ELISA_GTX10226-1_19011506_269.jpg "Anti-Cardiac Troponin I antibody [16A11] used in ELISA (ELISA). GTX10226")
![Anti-Cardiac Troponin I antibody [16A11] used in Western Blot (WB). GTX10226](/upload/website/prouct_img/normal/GTX10226/Cardiac-Troponin-I-antibody-16A11_Western blot_GTX10226-1_19011720_307.jpg "Anti-Cardiac Troponin I antibody [16A11] used in Western Blot (WB). GTX10226")