Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
Assay dependent |
Assay dependent |
Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
Reacts equally with free cardiac troponin I (cTnI) and cTnI forming complexes with other troponincomponents. Not affected by heparin, phosphorylation and oxidation. Recognizes an epitope locatedbetween amino acid residues 41 and 49 of cTnI. No cross-reactivity with skeletal muscle troponin I.Reactive with cTnI from human, monkey, bovine, porcine, goat, canine, rabbit, feline, rat, mouse and fish.
Form
Liquid
Buffer
20mM Tris-HCl pH7.5, 150mM Sodium Chloride
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
Concentration
1.40 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Native Troponin Complex
Purification
Protein A purified
Conjugation
Biotin
RRID
AB_11167426
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Synonyms
troponin I3, cardiac type , CMD1FF , CMD2A , CMH7 , RCM1 , TNNC1 , cTnI
Background
Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq, Jul 2008]
Database
Research Area