GeneTex
United States (US)

Cardiac Troponin I antibody [560]

Cat No. GTX38795

Host Mouse
Clonality Monoclonal
Clone Name 560
Isotype IgG1
Application WB, Apuri, IA, sELISA
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
Apuri Assay dependent
IA Assay dependent
sELISA Assay dependent

Note :

sELISA
Capture: GTX38795, Detection: GTX38796.

Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Specificity/Sensitivity

Reacts equally with free cardiac troponin I (cTnl) and cTnl forming complexes with other troponincomponents. Not effected by heparin, phosphorylation and oxidation. Recognizes an epitope locatedbetween amino acid residues 83 and 93 of cTnI. No cross reactivity with skeletal muscle troponin I.
PROPERTIES

Form

Liquid

Buffer

PBS pH 7.4, 0.09% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

6.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human Troponin Complex

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Troponin I3, Cardiac Type,Cmd1Ff,Cmd2A,Cmh7,Rcm1,Tnnc1,Ctni,Tnni3

Background

Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq, Jul 2008]

Database

Research Area

Package List Price ($)
$ 329