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Cardiac Troponin I antibody [8E10]

Anti-Cardiac Troponin I antibody [8E10] used in ELISA (ELISA). GTX10232
Anti-Cardiac Troponin I antibody [8E10] used in Western Blot (WB). GTX10232

Cat No. GTX10232

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 8E10

Isotype

 IgG1

Application

 WB, IP, ELISA, Immunoassay, IHC, Purification

Reactivity

 Human, Rabbit, Goat, Bovine, Cat, Dog, Pig
Package
200 μg ($259)
See all Cardiac Troponin I products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
IP Assay dependent
ELISA Assay dependent
Immunoassay Assay dependent
IHC Assay dependent
Purification Assay dependent
Not tested in other applications.

Calculated MW

24.01 kDa. ( Note )

Specificity/Sensitivity

No cross-reactivity with skeletal muscle TnI.

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.4, 0.09 % sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4º C.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Free human cardiac troponin I, native human cardiac troponin complex, or human cardiac troponin I peptides.

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Troponin I3, Cardiac Type , Cmd1Ff , Cmd2A , Cmh7 , Rcm1 , Tnnc1 , Ctni , Tnni3

Background

Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Cardiac Troponin I antibody [8E10] used in ELISA (ELISA). GTX10232

GTX10232 ELISA Image

Titration of human serum in two albumin sandwich immunoassays. Human Albumin antibody [15C7] (GTX10241) for capture and Human Albumin antibody [1A9] (GTX10242) for detection

Anti-Cardiac Troponin I antibody [8E10] used in Western Blot (WB). GTX10232

GTX10232 WB Image

Recognition of the dephospho- and phospho- forms of human cardiac troponin I. A. Cardiac Troponin I antibody [10B11] (GTX10225); B. Cardiac Troponin I antibody [8E10] (GTX10232)

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 259