GeneTex
United States (US)

Cardiac Troponin I antibody [B159M]

Cat No. GTX38832

Host Mouse
Clonality Monoclonal
Clone Name B159M
Isotype IgG1
Application WB, IP, Apuri, IA, IHC
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
IP Assay dependent
Apuri Assay dependent
IA Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

24 kDa kDa. ( Note )

Specificity/Sensitivity

Reacts with free cardiac troponin I (cTnI). Reacts weakly with cTnI forming complexes with other troponin components.Not affected by phosphorylation. Recognizes an epitope located between amino acid residues 148 and 158 of cTnI. Nocross-reactivity with skeletal muscle troponin I.
PROPERTIES

Form

Liquid

Buffer

PBS pH 7.4, 0.1% sodium azide

Concentration

4.30mg/ml

Antigen Species

Human

Immunogen

Free human cTnI and native human cTn complex

Purification

Protein A purified

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

TNNI3 Antibody , RCM1 Antibody , TNNC1 Antibody , CMD1FF Antibody , CTNI Antibody , CMD2A Antibody , CMH7 Antibody

Background

Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq, Jul 2008]
Show More +

Database

Research Area