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Cardiac Troponin I antibody [HL1489]

Recombinant antibody Comparison Orthogonal
Anti-Cardiac Troponin I antibody [HL1489] used in Western Blot (WB). GTX636963
Anti-Cardiac Troponin I antibody [HL1489] used in Western Blot (WB). GTX636963
Anti-Cardiac Troponin I antibody [HL1489] used in Western Blot (WB). GTX636963
Anti-Cardiac Troponin I antibody [HL1489] used in IHC (Paraffin sections) (IHC-P). GTX636963
Anti-Cardiac Troponin I antibody [HL1489] used in IHC (Paraffin sections) (IHC-P). GTX636963
Anti-Cardiac Troponin I antibody [HL1489] used in IHC (Paraffin sections) (IHC-P). GTX636963

Cat. No. GTX636963

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1489

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat, Cat
Package
100 μl ($399),
25 μl ($169)
See all Cardiac Troponin I products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human Cardiac Troponin I recombinant protein

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

troponin I3, cardiac type , CMD1FF , CMD2A , CMH7 , RCM1 , TNNC1 , cTnI

Background

Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Cardiac Troponin I antibody [HL1489] used in Western Blot (WB). GTX636963

GTX636963 WB Image

Comparison Orthogonal

Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membranes were blotted with Cardiac Troponin I antibody [HL1489] (GTX636963) diluted at 1:10000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-Cardiac Troponin I antibody [HL1489] used in Western Blot (WB). GTX636963

GTX636963 WB Image

Human tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Cardiac Troponin I antibody [HL1489] (GTX636963) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cardiac Troponin I antibody [HL1489] used in Western Blot (WB). GTX636963

GTX636963 WB Image

Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Cardiac Troponin I antibody [HL1489] (GTX636963) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cardiac Troponin I antibody [HL1489] used in IHC (Paraffin sections) (IHC-P). GTX636963

GTX636963 IHC-P Image

Cardiac Troponin I antibody [HL1489] detects Cardiac Troponin I protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
Cardiac Troponin I stained by Cardiac Troponin I antibody [HL1489] (GTX636963) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Cardiac Troponin I antibody [HL1489] used in IHC (Paraffin sections) (IHC-P). GTX636963

GTX636963 IHC-P Image

Cardiac Troponin I antibody [HL1489] detects Cardiac Troponin I protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat heart.
Cardiac Troponin I stained by Cardiac Troponin I antibody [HL1489] (GTX636963) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Cardiac Troponin I antibody [HL1489] used in IHC (Paraffin sections) (IHC-P). GTX636963

GTX636963 IHC-P Image

Cardiac Troponin I antibody [HL1489] detects Cardiac Troponin I protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded cat heart.
Cardiac Troponin I stained by Cardiac Troponin I antibody [HL1489] (GTX636963) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations