GeneTex
United States (US)

Cardiac Troponin I antibody [M18]

Cat No. GTX10236

Host Mouse
Clonality Monoclonal
Clone Name M18
Isotype IgG1
Application WB, ELISA, Apuri
Reactivity Human
APPLICATION

Application Note

Recommended pairs for ELISA:
19C7 (GTX28289) + M18 (this antibody) mixture for capture and MF4 (GTX10239) + 16A11 (GTX10226) mixture for detection
M18 (this antibody) + 560 (GTX38759) mixture for capture and MF4 (GTX10239) + 19C7 (GTX28289) mixture for detection
M18 (GTX10236) + 560 (GTX38759) mixture for capture and 19C7 (GTX28289) + 267 (GTX14530) mixture for detection

Calculated MW

24 kDa. ( Note )

Specificity/Sensitivity

This antibody is reacting with free cardiac troponin I (cTnI) and cTnI forming complexes with other troponin components (In the presence of 5 mM EDTA). It is not affected by heparin, phosphorylation, oxidation and troponin complex formation.This antibody does not cross-react with skeletal muscle troponin I. Epitope : 18-28 of human cardiac Troponin I.
PROPERTIES

Form

Liquid

Buffer

PBS pH 7.4, 0.09 % sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Antigen Species

Human

Immunogen

Free human cardiac troponin I, native human cardiac troponin complex, or human cardiac troponin I peptides.

Purification

Protein A purified
Purity tested by electrophoresis.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Background

Troponin I is part of a heteromeric complex playing an important role in the regulation of skeletal and cardiac muscle contraction. It consists of three subunits, troponin I (TnI), troponin T (TnT) and troponin C (TnC). Each subunit is responsible for part of troponin complex function. E.g. TnI inhibits ATP-ase activity of acto-myosin. TnT and TnI are presented in cardiac muscles in different forms than in skeletal muscles. Only one tissue-specific isoform of TnI is described for cardiac muscle tissue (cTnI). cTnI is expressed only in myocardium.

Database

Research Area

Package List Price ($)
$ 289