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Cardiac Troponin I antibody [N1C3-2]

Anti-Cardiac Troponin I antibody [N1C3-2] used in Western Blot (WB). GTX112770
Anti-Cardiac Troponin I antibody [N1C3-2] used in Western Blot (WB). GTX112770
Anti-Cardiac Troponin I antibody [N1C3-2] used in Western Blot (WB). GTX112770

Cat. No. GTX112770

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Cardiac Troponin I products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:20000
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Positive Control

Mouse heart , A431 , Molt-4 , rat heart

Predict Reactivity

Bovine, Cat, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cardiac Troponin I. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952325

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

troponin I3, cardiac type , CMD1FF , CMD2A , CMH7 , RCM1 , TNNC1 , cTnI

Background

Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Cardiac Troponin I antibody [N1C3-2] used in Western Blot (WB). GTX112770

GTX112770 WB Image

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Cardiac Troponin I antibody [N1C3-2] (GTX112770) diluted at 1:10000.

Anti-Cardiac Troponin I antibody [N1C3-2] used in Western Blot (WB). GTX112770

GTX112770 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
B: Molt-4 (GTX27912)
12% SDS PAGE
GTX112770 diluted at 1:1000

Anti-Cardiac Troponin I antibody [N1C3-2] used in Western Blot (WB). GTX112770

GTX112770 WB Image

Sample (50 ug of whole cell lysate)
A: mouse heart
12% SDS PAGE
GTX112770 diluted at 1:10000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169